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@article{Androniciuc:2024:10.17912/micropub.biology.001210,
author = {Androniciuc, A-M and Tate, EW and Vincent, J-P},
doi = {10.17912/micropub.biology.001210},
journal = {MicroPubl Biol},
title = {Engineering of TurboID-Wingless for the identification of Wingless interactors through in vivo proximity labelling.},
url = {http://dx.doi.org/10.17912/micropub.biology.001210},
volume = {2024},
year = {2024}
}
TY - JOUR
AB - Wnt signalling coordinates growth and cell fate decisions during development and mis-regulation of Wnt signalling in adults is associated with a range of conditions, including cancer and neurodegenerative diseases. Therefore, means of modulating Wnt proteins and/or cofactors could have significant therapeutic potential. As a first step towards enumerating the Wnt interactome, we devised an in vivo proximity labelling strategy to identify proteins that interact with Wingless (Wg), the main Drosophila Wnt. We engineered the wingless locus to express a functional TurboID-Wg fusion at endogenous levels and identified in vivo interactors by streptavidin pull-down from embryos, followed by mass spectrometry. Further analysis may in future extend the screen coverage and deliver functional validation of the newly identified interactors.
AU - Androniciuc,A-M
AU - Tate,EW
AU - Vincent,J-P
DO - 10.17912/micropub.biology.001210
PY - 2024///
TI - Engineering of TurboID-Wingless for the identification of Wingless interactors through in vivo proximity labelling.
T2 - MicroPubl Biol
UR - http://dx.doi.org/10.17912/micropub.biology.001210
UR - https://www.ncbi.nlm.nih.gov/pubmed/38872844
VL - 2024
ER -
Prof. Ed Tate
GSK Chair in Chemical Biology
Department of Chemistry
Molecular Sciences Research Hub, White City Campus,
82 Wood Lane, London, W12 0BZ
e.tate@imperial.ac.uk
Tel: +44 (0)20 759 + ext 43752 or 45821