BibTex format
@article{Ehsan:2021:10.1021/acschembio.1c00578,
author = {Ehsan, M and Wang, H and Cecchetti, C and Mortensen, JS and Du, Y and Hariharan, P and Nygaard, A and Lee, HJ and Ghani, L and Guan, L and Loland, CJ and Byrne, B and Kobilka, BK and Chae, PS},
doi = {10.1021/acschembio.1c00578},
journal = {ACS Chemical Biology},
pages = {1779--1790},
title = {Maltose-bis(hydroxymethyl)phenol (MBPs) and Maltosetris(hydroxymethyl)phenol (MTPs) Amphiphiles for Membrane Protein Stability},
url = {http://dx.doi.org/10.1021/acschembio.1c00578},
volume = {16},
year = {2021}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Membrane protein structures provide a fundamental understanding of their molecular actions and are of importance for drug development. Detergents are widely used to solubilize, stabilize, and crystallize membrane proteins, but membrane proteins solubilized in conventional detergents are prone to denaturation and aggregation. Thus, developing novel detergents with enhanced efficacy for protein stabilization remains important. We report herein the design and synthesis of a class of phenol-derived maltoside detergents. Using two different linkers, we prepared two sets of new detergents, designated maltose-bis(hydroxymethyl)phenol (MBPs) and maltose-tris(hydroxymethyl)phenol (MTPs). The evaluation of these detergents with three transporters and two G-protein coupled receptors allowed us to identify a couple of new detergents (MBP-C9 and MTP-C12) that consistently conferred enhanced stability to all tested proteins compared to a gold standard detergent (DDM). Furthermore, the data analysis based on the detergent structures provides key detergent features responsible for membrane protein stabilization that together will facilitate the future design of novel detergents.
AU - Ehsan,M
AU - Wang,H
AU - Cecchetti,C
AU - Mortensen,JS
AU - Du,Y
AU - Hariharan,P
AU - Nygaard,A
AU - Lee,HJ
AU - Ghani,L
AU - Guan,L
AU - Loland,CJ
AU - Byrne,B
AU - Kobilka,BK
AU - Chae,PS
DO - 10.1021/acschembio.1c00578
EP - 1790
PY - 2021///
SN - 1554-8929
SP - 1779
TI - Maltose-bis(hydroxymethyl)phenol (MBPs) and Maltosetris(hydroxymethyl)phenol (MTPs) Amphiphiles for Membrane Protein Stability
T2 - ACS Chemical Biology
UR - http://dx.doi.org/10.1021/acschembio.1c00578
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000697343400017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://pubs.acs.org/doi/10.1021/acschembio.1c00578
VL - 16
ER -