In this section
@article{Han:2021:10.1038/s41598-021-02742-w,
author = {Han, P and Go, MK and Chow, JY and Xue, B and Lim, YP and Crone, MA and Storch, M and Freemont, PS and Yew, WS},
doi = {10.1038/s41598-021-02742-w},
journal = {Scientific Reports},
pages = {1--10},
title = {A high-throughput pipeline for scalable kit-free RNA extraction},
url = {http://dx.doi.org/10.1038/s41598-021-02742-w},
volume = {11},
year = {2021}
}
TY - JOUR
AB - An overreliance on commercial, kit-based RNA extraction in the molecular diagnoses of infectious disease presents a challenge in the event of supply chain disruptions and can potentially hinder testing capacity in times of need. In this study, we adapted a well-established, robust TRIzol-based RNA extraction protocol into a high-throughput format through miniaturization and automation. The workflow was validated by RT-qPCR assay for SARS-CoV-2 detection to illustrate its scalability without interference to downstream diagnostic sensitivity and accuracy. This semi-automated, kit-free approach offers a versatile alternative to prevailing integrated solid-phase RNA extraction proprietary systems, with the added advantage of improved cost-effectiveness for high volume acquisition of quality RNA whether for use in clinical diagnoses or for diverse molecular applications.
AU - Han,P
AU - Go,MK
AU - Chow,JY
AU - Xue,B
AU - Lim,YP
AU - Crone,MA
AU - Storch,M
AU - Freemont,PS
AU - Yew,WS
DO - 10.1038/s41598-021-02742-w
EP - 10
PY - 2021///
SN - 2045-2322
SP - 1
TI - A high-throughput pipeline for scalable kit-free RNA extraction
T2 - Scientific Reports
UR - http://dx.doi.org/10.1038/s41598-021-02742-w
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000725094400048&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://www.nature.com/articles/s41598-021-02742-w
VL - 11
ER -