BibTex format
@article{Rattu:2021:10.1016/j.csbj.2021.11.014,
author = {Rattu, P and Glencross, F and Mader, SL and Skylaris, C-K and Matthews, SJ and Rouse, SL and Khalid, S},
doi = {10.1016/j.csbj.2021.11.014},
journal = {Computational and Structural Biotechnology Journal},
pages = {6417--6430},
title = {Atomistic level characterisation of ssDNA translocation through the E. coli proteins CsgG and CsgF for nanopore sequencing},
url = {http://dx.doi.org/10.1016/j.csbj.2021.11.014},
volume = {19},
year = {2021}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Two proteins of the Escherichia coli membrane protein complex, CsgG and CsgF, are studied as proteinaceous nanopores for DNA sequencing. It is highly desirable to control the DNA as it moves through the pores, this requires characterisation of DNA translocation and subsequent optimization of the pores. In order to inform protein engineering to improve the pores, we have conducted a series of molecular dynamics simulations to characterise the mechanical strength and conformational dynamics of CsgG and the CsgG-CsgF complex and how these impact ssDNA, water and ion movement. We find that the barrel of CsgG is more susceptible to damage from external electric fields compared to the protein vestibule. Furthermore, the presence of CsgF within the CsgG-CsgF complex enables the complex to withstand higher electric fields. We find that the eyelet loops of CsgG play a key role in both slowing the translocation rate of DNA and modulating the conductance of the pore. CsgF also impacts the DNA translocation rate, but to a lesser degree than CsgG.
AU - Rattu,P
AU - Glencross,F
AU - Mader,SL
AU - Skylaris,C-K
AU - Matthews,SJ
AU - Rouse,SL
AU - Khalid,S
DO - 10.1016/j.csbj.2021.11.014
EP - 6430
PY - 2021///
SN - 2001-0370
SP - 6417
TI - Atomistic level characterisation of ssDNA translocation through the E. coli proteins CsgG and CsgF for nanopore sequencing
T2 - Computational and Structural Biotechnology Journal
UR - http://dx.doi.org/10.1016/j.csbj.2021.11.014
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000731411300004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
VL - 19
ER -