In this section
@article{Kennedy:2022:10.1049/enb2.12024,
author = {Kennedy, A and Griffin, G and Freemont, PS and Polizzi, KM and Moore, SJ},
doi = {10.1049/enb2.12024},
journal = {Eng Biol},
pages = {62--68},
title = {A curcumin direct protein biosensor for cell-free prototyping.},
url = {http://dx.doi.org/10.1049/enb2.12024},
volume = {6},
year = {2022}
}
TY - JOUR
AB - In synthetic biology, biosensors are routinely coupled with a gene expression system for detecting small molecules and physical signals. We reveal a fluorescent complex, based on the interaction of an Escherichia coli double bond reductase (EcCurA), as a detection unit with its substrate curcumin-we call this a direct protein (DiPro) biosensor. Using a cell-free synthetic biology approach, we use the EcCurA DiPro biosensor to fine tune 10 reaction parameters (cofactor, substrate, and enzyme levels) for cell-free curcumin biosynthesis, assisted through acoustic liquid handling robotics. Overall, we increase EcCurA-curcumin DiPro fluorescence within cell-free reactions by 78-fold. This finding adds to the growing family of protein-ligand complexes that are naturally fluorescent and potentially exploitable for a range of applications, including medical imaging to engineering high-value chemicals.
AU - Kennedy,A
AU - Griffin,G
AU - Freemont,PS
AU - Polizzi,KM
AU - Moore,SJ
DO - 10.1049/enb2.12024
EP - 68
PY - 2022///
SP - 62
TI - A curcumin direct protein biosensor for cell-free prototyping.
T2 - Eng Biol
UR - http://dx.doi.org/10.1049/enb2.12024
UR - https://www.ncbi.nlm.nih.gov/pubmed/36969103
VL - 6
ER -