In this section
@article{Ibarra-Chavez:2023:10.1002/advs.202301643,
author = {Ibarra-Chavez, R and Reboud, J and Penades, JR and Cooper, JM},
doi = {10.1002/advs.202301643},
journal = {Advanced Science},
title = {Phage-inducible chromosomal islands as a diagnostic platform to capture and detect bacterial pathogens},
url = {http://dx.doi.org/10.1002/advs.202301643},
volume = {10},
year = {2023}
}
TY - JOUR
AB - Phage-inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user-friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL−1. In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differentiate between dead and live organisms, this approach enables visual sensing of viable pathogens only, through the expression of a reporter gene encoded in the PICI. The approach extends diagnostic sensing mechanisms beyond cell-free synthetic biology strategies, enabling new synthetic biology/biosensing toolkits.
AU - Ibarra-Chavez,R
AU - Reboud,J
AU - Penades,JR
AU - Cooper,JM
DO - 10.1002/advs.202301643
PY - 2023///
SN - 2198-3844
TI - Phage-inducible chromosomal islands as a diagnostic platform to capture and detect bacterial pathogens
T2 - Advanced Science
UR - http://dx.doi.org/10.1002/advs.202301643
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:001016069300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR - https://onlinelibrary.wiley.com/doi/10.1002/advs.202301643
VL - 10
ER -