In this section
@article{Teh:2023:10.1128/spectrum.01481-23,
author = {Teh, WK and Ding, Y and Gubellini, F and Filloux, A and Poyart, C and Givskov, M and Dramsi, S},
doi = {10.1128/spectrum.01481-23},
journal = {Microbiology Spectrum},
title = {Characterization of TelE, a T7SS LXG effector exhibiting a conserved C-terminal glycine zipper motif required for toxicity},
url = {http://dx.doi.org/10.1128/spectrum.01481-23},
volume = {11},
year = {2023}
}
TY - JOUR
AB - Streptococcus gallolyticus subsp. gallolyticus (SGG) is an opportunistic bacterial pathogen strongly associated with colorectal cancer. Here, through comparative genomics analysis, we demonstrated that the genetic locus encoding the type VIIb secretion system (T7SSb) machinery is uniquely present in SGG in two different arrangements. SGG UCN34 carrying the most prevalent T7SSb genetic arrangement was chosen as the reference strain. To identify the effectors secreted by this secretion system, we inactivated the essC gene encoding the motor of this machinery. A comparison of the proteins secreted by UCN34 wild type and its isogenic ΔessC mutant revealed six T7SSb effector proteins, including the expected WXG effector EsxA and three LXG-containing proteins. In this work, we characterized an LXG-family toxin named herein TelE promoting the loss of membrane integrity. Seven homologs of TelE harboring a conserved glycine zipper motif at the C terminus were identified in different SGG isolates. Scanning mutagenesis of this motif showed that the glycine residue at position 470 was crucial for TelE membrane destabilization activity. TelE activity was antagonized by a small protein TipE belonging to the DUF5085 family. Overall, we report herein a unique SGG T7SSb effector exhibiting a toxic activity against nonimmune bacteria.
AU - Teh,WK
AU - Ding,Y
AU - Gubellini,F
AU - Filloux,A
AU - Poyart,C
AU - Givskov,M
AU - Dramsi,S
DO - 10.1128/spectrum.01481-23
PY - 2023///
SN - 2165-0497
TI - Characterization of TelE, a T7SS LXG effector exhibiting a conserved C-terminal glycine zipper motif required for toxicity
T2 - Microbiology Spectrum
UR - http://dx.doi.org/10.1128/spectrum.01481-23
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:001026125400001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR - https://journals.asm.org/doi/10.1128/spectrum.01481-23
VL - 11
ER -