BibTex format
@article{McKenna:2023:10.1016/j.bbapap.2023.140946,
author = {McKenna, S and Aylward, F and Miliara, X and Lau, RJ and Huemer, CB and Giblin, SP and Huse, KK and Liang, M and Reeves, L and Pearson, M and Xu, Y and Rouse, SL and Pease, JE and Sriskandan, S and Kagawa, TF and Cooney, J and Matthews, S},
doi = {10.1016/j.bbapap.2023.140946},
journal = {BBA: Proteins and Proteomics},
pages = {1--11},
title = {The protease associated (PA) domain in ScpA from Streptococcus pyogenes plays a role in substrate recruitment},
url = {http://dx.doi.org/10.1016/j.bbapap.2023.140946},
volume = {1871},
year = {2023}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Annually, over 18 million disease cases and half a million deaths worldwide are estimated to be caused by Group A Streptococcus. ScpA (or C5a peptidase) is a well characterised member of the cell enveleope protease family, which possess a S8 subtilisin-like catalytic domain and a shared multi-domain architecture. ScpA cleaves complement factors C5a and C3a, impairing the function of these critical anaphylatoxins and disrupts complement-mediated innate immunity. Although the high resolution structure of ScpA is known, the details of how it recognises its substrate are only just emerging. Previous studies have identified a distant exosite on the 2nd fibronectin domain that plays an important role in recruitment via an interaction with the substrate core. Here, using a combination of solution NMR spectroscopy, mutagenesis with functional assays and computational approaches we identify a second exosite within the protease-associated (PA) domain. We propose a model in which the PA domain assists optimal delivery of the substrate's C terminus to the active site for cleavage.
AU - McKenna,S
AU - Aylward,F
AU - Miliara,X
AU - Lau,RJ
AU - Huemer,CB
AU - Giblin,SP
AU - Huse,KK
AU - Liang,M
AU - Reeves,L
AU - Pearson,M
AU - Xu,Y
AU - Rouse,SL
AU - Pease,JE
AU - Sriskandan,S
AU - Kagawa,TF
AU - Cooney,J
AU - Matthews,S
DO - 10.1016/j.bbapap.2023.140946
EP - 11
PY - 2023///
SN - 1570-9639
SP - 1
TI - The protease associated (PA) domain in ScpA from Streptococcus pyogenes plays a role in substrate recruitment
T2 - BBA: Proteins and Proteomics
UR - http://dx.doi.org/10.1016/j.bbapap.2023.140946
UR - https://www.ncbi.nlm.nih.gov/pubmed/37562488
UR - https://www.sciencedirect.com/science/article/pii/S1570963923000602
VL - 1871
ER -