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@article{Millership:2024:10.1007/978-1-0716-3491-2_8,
author = {Millership, C and Gründling, A},
doi = {10.1007/978-1-0716-3491-2_8},
journal = {Methods Mol Biol},
pages = {95--106},
title = {Type I Lipoteichoic Acid (LTA) Detection by Western Blot.},
url = {http://dx.doi.org/10.1007/978-1-0716-3491-2_8},
volume = {2727},
year = {2024}
}
TY - JOUR
AB - Type I lipoteichoic acid (LTA) is a glycerol phosphate polymer found in the cell envelope of diverse Gram-positive bacteria including Staphylococcus aureus, Bacillus subtilis, and Listeria monocytogenes. The polymer is linked by a lipid anchor to the outer leaflet of the bacterial membrane and in some bacteria can also be shed and detected in the culture supernatant. Here, we describe a simple and rapid western blot method for the detection of Type I LTA in bacterial cell extracts and culture supernatant fractions using a polyglycerol phosphate specific monoclonal LTA antibody.
AU - Millership,C
AU - Gründling,A
DO - 10.1007/978-1-0716-3491-2_8
EP - 106
PY - 2024///
SP - 95
TI - Type I Lipoteichoic Acid (LTA) Detection by Western Blot.
T2 - Methods Mol Biol
UR - http://dx.doi.org/10.1007/978-1-0716-3491-2_8
UR - https://www.ncbi.nlm.nih.gov/pubmed/37815711
VL - 2727
ER -