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@article{Rismondo:2024:10.1007/978-1-0716-3491-2_9,
author = {Rismondo, J and Gründling, A},
doi = {10.1007/978-1-0716-3491-2_9},
journal = {Methods Mol Biol},
pages = {107--124},
title = {Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spectroscopy.},
url = {http://dx.doi.org/10.1007/978-1-0716-3491-2_9},
volume = {2727},
year = {2024}
}
TY - JOUR
AB - Type I lipoteichoic acid (LTA) is a glycerol phosphate polymer found in the cell envelope of diverse Gram-positive bacteria. The glycerol phosphate backbone is often further decorated with D-alanine and/or sugar residues. Here, we provide details of a 1-butanol extraction and purification method of type I LTA by hydrophobic interaction chromatography. The protocol has been adapted from methods originally described by Fischer et al. (Eur J Biochem 133:523-530, 1983) and further optimized by Morath et al. (J Exp Med 193:393-397, 2001). We also present information on a 2D nuclear magnetic resonance (NMR) analysis method to gain chemical and structural information of the purified LTA material.
AU - Rismondo,J
AU - Gründling,A
DO - 10.1007/978-1-0716-3491-2_9
EP - 124
PY - 2024///
SP - 107
TI - Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spectroscopy.
T2 - Methods Mol Biol
UR - http://dx.doi.org/10.1007/978-1-0716-3491-2_9
UR - https://www.ncbi.nlm.nih.gov/pubmed/37815712
VL - 2727
ER -