BibTex format

author = {Fallesen, T and Roostalu, J and Duellberg, C and Pruessner, G and Surrey, T},
doi = {10.1016/j.bpj.2017.09.006},
journal = {Biophysical Journal},
pages = {2055--2067},
title = {Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement},
url = {},
volume = {113},
year = {2017}

RIS format (EndNote, RefMan)

AB - Most kinesin motors move in only one direction along microtubules. Members of the kinesin-5 subfamily were initially described as unidirectional plus-end-directed motors and shown to produce piconewton forces. However, some fungal kinesin-5 motors are bidirectional. The force production of a bidirectional kinesin-5 has not yet been measured. Therefore, it remains unknown whether the mechanism of the unconventional minus-end-directed motility differs fundamentally from that of plus-end-directed stepping. Using force spectroscopy, we have measured here the forces that ensembles of purified budding yeast kinesin-5 Cin8 produce in microtubule gliding assays in both plus- and minus-end direction. Correlation analysis of pause forces demonstrated that individual Cin8 molecules produce additive forces in both directions of movement. In ensembles, Cin8 motors were able to produce single-motor forces up to a magnitude of ∼1.5 pN. Hence, these properties appear to be conserved within the kinesin-5 subfamily. Force production was largely independent of the directionality of movement, indicating similarities between the motility mechanisms for both directions. These results provide constraints for the development of models for the bidirectional motility mechanism of fission yeast kinesin-5 and provide insight into the function of this mitotic motor.
AU - Fallesen,T
AU - Roostalu,J
AU - Duellberg,C
AU - Pruessner,G
AU - Surrey,T
DO - 10.1016/j.bpj.2017.09.006
EP - 2067
PY - 2017///
SN - 0006-3495
SP - 2055
TI - Ensembles of Bidirectional Kinesin Cin8 Produce Additive Forces in Both Directions of Movement
T2 - Biophysical Journal
UR -
UR -
VL - 113
ER -