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• Journal article
  Rafieenia R, Fu J, Hapeta P, Storch M, Ledesma-Amaro Ret al., 2026,

  Advancing arabinose-based bioproduction in Yarrowia lipolytica by integrating metabolic engineering and adaptive laboratory evolution

  , Metabolic Engineering, Vol: 94, Pages: 15-23, ISSN: 1096-7176

  The oleaginous yeast, Yarrowia lipolytica has gained interest as a biotechnological chassis to produce foods, chemicals, pharmaceuticals, and biofuels. To reduce production costs and sustainability, inexpensive and abundant feedstocks such as lignocellulose must be used for bioproduction. Since lignocellulosic biomass contains components that cannot be utilised by Y. lipolytica, it is important to use engineering biology to enable their utilisation. L-arabinose is the second most abundant pentose in lignocellulose after xylose. However, it has received much less attention than xylose as a bioresource. In the present study, we first engineered Y. lipolytica to grow on L-arabinose as the sole carbon source. We used several wild-type and engineered strains to express the multigene arabinose cassette. Second, we used adaptive laboratory evolution to improve the utilisation of arabinose by the engineered strains. Third, we enabled the production of β-carotene from arabinose by expressing a β-carotene cassette in the evolved strain. Using minimal YNB medium supplemented with 20 g/l of arabinose as the sole carbon source resulted in the complete utilisation of L-arabinose within 120 h. In bioreactors, a β-carotene production of 418.89 mg/l was achieved with the complete utilisation of 60 g/l of L-arabinose. This study is the first to engineer L-arabinose utilisation in Y. lipolytica, opening new avenues for biomanufacturing using alternative carbon sources.

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• Journal article
  Sun T, Sun M-L, Lin L, Gao J, Ledesma-Amaro R, Wang K, Ji X-Jet al., 2026,

  Combining multiplex metabolic engineering with adaptive evolution strategies for high-level succinic acid production in Yarrowia lipolytica

  , Synthetic and Systems Biotechnology, Vol: 11, Pages: 48-58, ISSN: 2405-805X

  Succinic acid, an essential platform chemical with extensive utility in biodegradable materials, pharmaceuticals, and the food industry, faces challenges of high energy consumption and environmental pollution in traditional chemical synthesis. Here, we employed multiplex metabolic engineering and adaptive laboratory evolution to enhance succinic acid biosynthesis in Yarrowia lipolytica. By attenuating succinate dehydrogenase (Sdh) activity, mitigating by-product accumulation, and enhancing the succinate synthesis pathway, engineered strains showed efficient succinic acid production from glycerol. The titer reached 130.99 g/L under unregulated pH conditions, translating to a yield of 0.35 g/g and a productivity of 0.70 g/(L·h). Subsequently, transporter engineering and adaptive evolution strategies were applied to enhance glucose utilization for succinic acid synthesis, yielding an evolved strain that eliminated the growth lag phase and produced 106.68 g/L succinic acid from glucose, which translated to a yield of 0.32 g/g and a productivity of 0.64 g/(L·h). Additionally, transcriptomic analysis and inverse metabolic engineering revealed that 4-hydroxyphenylpyruvate dioxygenase (4-Hppd) in the tyrosine degradation pathway partially restored the growth of Sdh-deficient strains on glucose, offering new insights for subsequent succinic acid biomanufacturing using Y. lipolytica.

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• Journal article
  Wang Y, Jiang Q, Vorlaufer D, Bismarck A, Blaker J, Gresil Met al., 2026,

  Application of vitrimer-based sizing agent onto carbon fibres through thiol-ene photo-polymerisation

  , COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING, Vol: 202, ISSN: 1359-835X
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• Journal article
  Almousa HA, De Luca HG, Anthony DB, Greenhalgh ES, Bismarck A, Shaffer MSPet al., 2026,

  Uniform and scalable carbon nanotube growth on carbon fibers: Insights from experimental dynamic snapshots and computational fluid dynamics

  , Carbon, Vol: 248, ISSN: 0008-6223

  Carbon nanotube (CNT) grafted carbon fibers (CFs) are promising for multifunctional composites (CFRPs) but remain limited by scalability, non-uniform growth, and degradation of fiber tensile strength. This paper reports a continuous spool-to-spool chemical vapor deposition (CVD) process that achieves uniform CNT growth throughout 12k CF tows while preserving fiber tensile properties. The uniformity of CNT coverage, over meters of length and across thousands of fibers, was objectively established via a multi-length scale characterization protocol, combining machine learning-based SEM classification with macroscopic measurements of BET-based specific surface area (SSA) and gravimetric CNT content. Microscopic and macroscopic measurements are independently self-consistent. To understand and optimize CNT growth, a new dynamic snapshot method was developed and combined with steady-state computational fluid dynamics (CFD) modelling to resolve the spatial evolution of catalyst activation, nucleation, and CNT growth kinetics as a function of reactor temperature and species concentrations. These insights informed targeted modifications to gas flow and temperature conditions, enabling reproducible CNT growth at 550 °C. Under optimized CVD conditions, the CFs were grafted with a CNT corona of 850 nm in length, corresponding to a loading of 2.9 wt% on the fibers, which led to a ten-fold increase in SSA (5.35 m² g⁻¹). The process was shown to be stable for extended lengths (>50 m) and reproducible across multiple runs, establishing a scalable route for integrating CNT-grafted CFs into conventional manufacturing. This experimental-computational framework provides a rational approach toward high-performance multifunctional, hierarchical CFRPs.

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• Journal article
  Chen H, Peng H, Ellis T, Ledesma-Amaro Ret al., 2026,

  Programmable cell-cell adhesion in synthetic yeast communities for improved bioproduction.

  , Nat Chem Biol

  In multicellular systems, engineering-controlled cell-cell adhesion and metabolic interdependence are vital for developing complex functionalities. This study introduces a yeast synthetic toolbox for modular cell-cell adhesion and cocultures, aiming to overcome the limitations of existing approaches that lack genetic specificity and control. First, a model yeast strain 007Δ is created with seven main flocculation and agglutination genes removed, providing a clean background for synthetic adhesion systems. Then, three distinct adhesion pair systems-Strategy 1, Strategy 2.1 and Strategy 2.2-are established involving yeast flocculation and agglutination proteins and yeast surface display systems. In addition, a quantitative assessment is conducted on the adhesive specificity and strength, alongside the capability of synthetic adhesion to generate patterns. Finally, we successfully demonstrate enhanced bioproduction of the high-value food antioxidant, resveratrol, utilizing synthetic cocultures coupled with cell adhesion systems. We anticipate that this toolkit will emerge as a valuable resource for diverse applications in synthetic biology and biomanufacturing.

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• Journal article
  McKenzie J, Carter C, Jackson MM, Singanayagam A, Shah Aet al., 2026,

  Mechanisms driving immunopathogenesis of viral exacerbations in chronic respiratory disease.

  , Thorax

  BACKGROUND: Exacerbations are major causes of morbidity in individuals with chronic respiratory diseases such as chronic obstructive pulmonary disease, asthma and bronchiectasis. Increasing evidence implicates respiratory viruses as predominant triggers, though the underlying immunopathogenic mechanisms remain poorly understood. NARRATIVE: This review synthesises current knowledge on the interplay between viral pathogens at the airway epithelial barrier, including structural and immunological mechanisms that may dysregulate antiviral immunity in chronic respiratory diseases. Furthermore, we discuss how perturbations in the respiratory microbiome, characterised by reduced microbial diversity, can modulate host antiviral immune defences. CONCLUSIONS: Collectively, these interconnected factors create a permissive environment predisposing to viral infection and exacerbations in chronic respiratory diseases. Understanding the complex interactions between airway structure, interferon-mediated antiviral responses, inflammation and microbiota is essential for developing targeted therapies to effectively manage virus-induced exacerbations and reduce disease burden.

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• Journal article
  Reed AK, Mercier O, Behr J, Dave K, Dellgren G, Kotecha S, Kukreja J, Landry C, Levvey B, Lyster H, Morrissey O, Saatee S, Sanchez M, Schecter M, Walsh J, Virginia A, Gelzinis TA, Hoetzenecker K, Lindstedt S, Marczin N, Wilkey BJ, Fessler J, Bottiger B, Wille K, Nasir BS, Gomez-De-Antonio D, Cypel M, Martin AKet al., 2026,

  ISHLT Consensus Statement on the Perioperative use of ECLS in Lung Transplantation: Part I: Preoperative Considerations

  , JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 45, Pages: e1-e34, ISSN: 1053-2498
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  • Citations: 1
• Journal article
  Martin AK, Mercier O, Bottiger B, Cypel M, Fessler J, Gomez-De-Antonio D, Levvey B, Lyster H, Nasir B, Sanchez M, Dclinpsy, Wille K, Fritz AV, Gelzinis T, Hoetzenecker K, Dave K, Lindstedt S, Marczin N, Wilkey B, Schecter M, Walsh J, Morrissey O, Landry C, Saatee S, Kotecha S, Behr J, Kukreja J, Dellgren G, Reed AKet al., 2026,

  Author Insights on the ISHLT Perioperative Utilization of ECLS In Lung Transplantation Consensus

  , JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 45, Pages: 1-4, ISSN: 1053-2498
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• Journal article
  Martin AK, Mercier O, Bottiger B, Cypel M, Fessler J, Gomez-De-Antonio D, Levvey B, Lyster H, Nasir B, Sanchez M, Wille K, Fritz AV, Gelzinis T, Hoetzenecker K, Dave K, Lindstedt S, Marczin N, Wilkey B, Schecter M, Walsh J, Morrissey O, Landry C, Saatee S, Kotecha S, Behr J, Kukreja J, Dellgren G, Reed AKet al., 2026,

  ISHLT Consensus Statement on the Perioperative use of ECLS in Lung Transplantation: Part III: Postoperative Considerations

  , JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 45, Pages: e63-e81, ISSN: 1053-2498
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  • Citations: 3
• Journal article
  Martin AK, Mercier O, Fritz AV, Gelzinis TA, Hoetzenecker K, Lindstedt S, Marczin N, Wilkey BJ, Schecter M, Lyster H, Sanchez M, Walsh J, Morrissey O, Levvey B, Landry C, Saatee S, Kotecha S, Behr J, Kukreja J, Dellgren G, Fessler J, Bottiger B, Wille K, Dave K, Nasir BS, Gomez-De-Antonio D, Cypel M, Reed AKet al., 2026,

  ISHLT Consensus Statement on the Perioperative use of ECLS in Lung Transplantation: Part II: Intraoperative Considerations

  , JOURNAL OF HEART AND LUNG TRANSPLANTATION, Vol: 45, Pages: e35-e62, ISSN: 1053-2498
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  • Citations: 10

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