In this section
@unpublished{Pyle:2017:10.1101/206714,
author = {Pyle, E and Kalli, AC and Amillis, S and Hall, Z and Hanyaloglu, AC and Diallinas, G and Byrne, B and Politis, A},
doi = {10.1101/206714},
title = {Structural lipids enable the formation of functional oligomers of the eukaryotic purine symporter UapA},
url = {http://dx.doi.org/10.1101/206714},
year = {2017}
}
TY - UNPB
AB - <jats:title>Abstract</jats:title><jats:p>The role of membrane lipids in modulating eukaryotic transporter structure and function remains poorly understood. We used native mass spectrometry in combination with molecular dynamics simulations and<jats:italic>in vivo</jats:italic>analyses to investigate the roles of membrane lipids in the structure and transport activity of the purine transporter, UapA, from<jats:italic>Aspergillus nidulans</jats:italic>. We revealed that UapA exists mainly as a dimer and that two lipid molecules bind per UapA dimer. We identified three classes of phospholipids: phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) which co-purified with UapA. Delipidation of UapA caused dissociation of the dimer into individual protomers. Subsequent addition of PI or PE rescued the UapA dimer and allowed recovery of bound lipids, suggesting a central role of these lipids in stabilising the dimer. We predicted a putative lipid-binding site near the UapA dimer interface. Mutational analyses established that lipid binding at this site is essential for formation of functional UapA dimers. Our findings reveal unprecedented level of detail into the nature of UapA-lipid interactions and provide a framework for studying similar eukaryotic systems.</jats:p>
AU - Pyle,E
AU - Kalli,AC
AU - Amillis,S
AU - Hall,Z
AU - Hanyaloglu,AC
AU - Diallinas,G
AU - Byrne,B
AU - Politis,A
DO - 10.1101/206714
PY - 2017///
TI - Structural lipids enable the formation of functional oligomers of the eukaryotic purine symporter UapA
UR - http://dx.doi.org/10.1101/206714
UR - https://doi.org/10.1101/206714
ER -
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