In this section
@article{Park:2020:10.3389/fbioe.2019.00484,
author = {Park, Y-K and Ledesma-Amaro, R and Nicaud, J-M},
doi = {10.3389/fbioe.2019.00484},
journal = {Frontiers in Bioengineering and Biotechnology},
pages = {1--11},
title = {De novo biosynthesis of odd-chain fatty acids in yarrowia lipolytica enabled by modular pathway engineering},
url = {http://dx.doi.org/10.3389/fbioe.2019.00484},
volume = {7},
year = {2020}
}
TY - JOUR
AB - Microbial oils are regarded as promising alternatives to fossil fuels as concerns over environmental issues and energy production systems continue to mount. Odd-chain fatty acids (FAs) are a type of valuable lipid with various applications: they can serve as biomarkers, intermediates in the production of flavor and fragrance compounds, fuels, and plasticizers. Microorganisms naturally produce FAs, but such FAs are primarily even-chain; only negligible amounts of odd-chain FAs are generated. As a result, studies using microorganisms to produce odd-chain FAs have had limited success. Here, our objective was to biosynthesize odd-chain FAs de novo in Yarrowia lipolytica using inexpensive carbon sources, namely glucose, without any propionate supplementation. To achieve this goal, we constructed a modular metabolic pathway containing seven genes. In the engineered strain expressing this pathway, the percentage of odd-chain FAs out of total FAs was higher than in the control strain (3.86 vs. 0.84%). When this pathway was transferred into an obese strain, which had been engineered to accumulate large amounts of lipids, odd-chain fatty acid production was 7.2 times greater than in the control (0.05 vs. 0.36 g/L). This study shows that metabolic engineering research is making progress toward obtaining efficient cell factories that produce odd-chain FAs.
AU - Park,Y-K
AU - Ledesma-Amaro,R
AU - Nicaud,J-M
DO - 10.3389/fbioe.2019.00484
EP - 11
PY - 2020///
SN - 2296-4185
SP - 1
TI - De novo biosynthesis of odd-chain fatty acids in yarrowia lipolytica enabled by modular pathway engineering
T2 - Frontiers in Bioengineering and Biotechnology
UR - http://dx.doi.org/10.3389/fbioe.2019.00484
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000511332900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR - https://www.frontiersin.org/articles/10.3389/fbioe.2019.00484/full
VL - 7
ER -
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