BibTex format
@article{Davies:2022:10.1016/j.jcf.2021.04.015,
author = {Davies, J and Kos, R and Brinkman, P and Neerincx, A and Paff, T and Gerritsen, M and Lammers, A and Kraneveld, A and Heijerman, H and Janssens, H and Majoor, C and Weersink, E and Sterk, P and Haarman, E and Bos, L and Maitland-van, der Zee A and on, behalf of the Amsterdam Mucociliary Clearance Disease AMCD Research Group and the Amsterdam UMC Breath Research Group},
doi = {10.1016/j.jcf.2021.04.015},
journal = {Journal of Cystic Fibrosis},
pages = {e28--e34},
title = {Targeted exhaled breath analysis for detection of Pseudomonas aeruginosa in cystic fibrosis patients},
url = {http://dx.doi.org/10.1016/j.jcf.2021.04.015},
volume = {21},
year = {2022}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - BackgroundPseudomonas aeruginosa (PA) is an important respiratory pathogen for cystic fibrosis (CF) patients. Routine microbiology surveillance is time-consuming, and is best performed on expectorated sputum. As alternative, volatile organic compounds (VOCs) may be indicative of PA colonisation. In this study, we aimed to identify VOCs associated with PA in literature and perform targeted exhaled breath analysis to recognize PA positive CF patients non-invasively.MethodsThis study consisted of 1) a literature review to select VOCs of interest, and 2) a cross-sectional CF study. Definitions used: A) PA positive, PA culture at visit/chronically; B) PA free, no PA culture in ≥12 months. Exhaled VOCs were identified via quadrupole MS. The primary endpoint was the area under the receiver operating characteristics curve (AUROCC) of individual VOCs as well as combined VOCs against PA culture.Results241 VOCs were identified in literature, of which 56 were further evaluated, and 13 could be detected in exhaled breath in our cohort. Exhaled breath of 25 pediatric and 28 adult CF patients, PA positive (n=16) and free (n=28) was available. 3/13 VOCs were significantly (p<0.05) different between PA groups in children; none were in adults. Notably, a composite model based on 5 or 1 VOC(s) showed an AUROCC of 0.86 (CI 0.71–1.0) and 0.87 (CI 0.72–1.0) for adults and children, respectively.ConclusionsTargeted VOC analysis appears to discriminate children and adults with and without PA positive cultures with clinically acceptable sensitivity values.
AU - Davies,J
AU - Kos,R
AU - Brinkman,P
AU - Neerincx,A
AU - Paff,T
AU - Gerritsen,M
AU - Lammers,A
AU - Kraneveld,A
AU - Heijerman,H
AU - Janssens,H
AU - Majoor,C
AU - Weersink,E
AU - Sterk,P
AU - Haarman,E
AU - Bos,L
AU - Maitland-van,der Zee A
AU - on,behalf of the Amsterdam Mucociliary Clearance Disease AMCD Research Group and the Amsterdam UMC Breath Research Group
DO - 10.1016/j.jcf.2021.04.015
EP - 34
PY - 2022///
SN - 1569-1993
SP - 28
TI - Targeted exhaled breath analysis for detection of Pseudomonas aeruginosa in cystic fibrosis patients
T2 - Journal of Cystic Fibrosis
UR - http://dx.doi.org/10.1016/j.jcf.2021.04.015
UR - https://www.sciencedirect.com/science/article/pii/S1569199321001259?via%3Dihub
VL - 21
ER -