In this section
@article{Drury:2022:10.1101/2022.12.15.520564,
author = {Drury, F and Grover, M and Hintze, M and Saunders, J and Fasseas, MK and Constantinou, C and Barkoulas, M},
doi = {10.1101/2022.12.15.520564},
title = {A receptor tyrosine kinase regulated by the transcription factor VAB-3/PAX6 pairs with a pseudokinase to trigger immune signalling upon oomycete recognition in <i>C. elegans</i>},
url = {http://dx.doi.org/10.1101/2022.12.15.520564},
year = {2022}
}
TY - JOUR
AB - <jats:title>Summary</jats:title> <jats:p> Oomycetes were recently discovered as natural pathogens of <jats:italic>Caenorhabditis elegans</jats:italic> and pathogen recognition alone was shown to be sufficient to activate a protective transcriptional program in the host characterised by the expression of multiple <jats:italic>chitinase-like</jats:italic> ( <jats:italic>chil</jats:italic> ) genes. However, the molecular mechanisms underlying oomycete recognition in animals remain fully unknown. We performed here a forward genetic screen to uncover regulators of <jats:italic>chil</jats:italic> gene induction and found several independent loss-of-function alleles of <jats:italic>old-1</jats:italic> and <jats:italic>flor-1,</jats:italic> which encode receptor tyrosine kinases belonging to the <jats:italic>C. elegans</jats:italic> -specific KIN-16 family. We present evidence that OLD-1 is an active kinase mounting the immune response, and FLOR-1 a pseudokinase that is also required for the response and regulates the distribution of OLD-1 at the epidermal membrane. Interestingly, the <jats:italic>old-1</jats:italic> locus is adjacent to the <jats:italic>chil</jats:italic> genes in the nematode genome, thereby revealing a genetic cluster important for oomycete-resistance. Furthermore, we identify the VAB-3/PAX-6 transcription factor known for its role in visual system development to regulate <jats:italic>old-1</jats:italic> expression, and consequently the spatial pattern o
AU - Drury,F
AU - Grover,M
AU - Hintze,M
AU - Saunders,J
AU - Fasseas,MK
AU - Constantinou,C
AU - Barkoulas,M
DO - 10.1101/2022.12.15.520564
PY - 2022///
TI - A receptor tyrosine kinase regulated by the transcription factor VAB-3/PAX6 pairs with a pseudokinase to trigger immune signalling upon oomycete recognition in <i>C. elegans</i>
UR - http://dx.doi.org/10.1101/2022.12.15.520564
UR - https://doi.org/10.1101/2022.12.15.520564
ER -
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