In this section
@article{Adachi:2023:10.1371/journal.pgen.1010500,
author = {Adachi, H and Sakai, T and Harant, AO and Pai, H and Honda, K and Toghani, A and Claeys, J and Duggan, C and Bozkurt, T and Wu, C-H and Kamoun, S},
doi = {10.1371/journal.pgen.1010500},
journal = {PLoS Genetics},
title = {An atypical NLR protein modulates the NRC immune receptor network in <i>Nicotiana benthamiana</i>},
url = {http://dx.doi.org/10.1371/journal.pgen.1010500},
volume = {19},
year = {2023}
}
TY - JOUR
AB - The NRC immune receptor network has evolved in asterid plants from a pair of linked genes into a genetically dispersed and phylogenetically structured network of sensor and helper NLR (nucleotide-binding domain and leucine-rich repeat-containing) proteins. In some species, such as the model plant Nicotiana benthamiana and other Solanaceae, the NRC (NLR-REQUIRED FOR CELL DEATH) network forms up to half of the NLRome, and NRCs are scattered throughout the genome in gene clusters of varying complexities. Here, we describe NRCX, an atypical member of the NRC family that lacks canonical features of these NLR helper proteins, such as a functional N-terminal MADA motif and the capacity to trigger autoimmunity. In contrast to other NRCs, systemic gene silencing of NRCX in N. benthamiana markedly impairs plant growth resulting in a dwarf phenotype. Remarkably, dwarfism of NRCX silenced plants is partially dependent on NRCX paralogs NRC2 and NRC3, but not NRC4. Despite its negative impact on plant growth when silenced systemically, spot gene silencing of NRCX in mature N. benthamiana leaves doesn’t result in visible cell death phenotypes. However, alteration of NRCX expression modulates the hypersensitive response mediated by NRC2 and NRC3 in a manner consistent with a negative role for NRCX in the NRC network. We conclude that NRCX is an atypical member of the NRC network that has evolved to contribute to the homeostasis of this genetically unlinked NLR network.
AU - Adachi,H
AU - Sakai,T
AU - Harant,AO
AU - Pai,H
AU - Honda,K
AU - Toghani,A
AU - Claeys,J
AU - Duggan,C
AU - Bozkurt,T
AU - Wu,C-H
AU - Kamoun,S
DO - 10.1371/journal.pgen.1010500
PY - 2023///
SN - 1553-7390
TI - An atypical NLR protein modulates the NRC immune receptor network in <i>Nicotiana benthamiana</i>
T2 - PLoS Genetics
UR - http://dx.doi.org/10.1371/journal.pgen.1010500
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000937212900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
VL - 19
ER -
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