In this section
@article{Arst:1980:10.1099/00221287-118-2-443,
author = {Arst, HN and Scazzocchio, C},
doi = {10.1099/00221287-118-2-443},
journal = {Journal of General Microbiology},
pages = {443--454},
title = {A method for monitoring macromolecular synthesis in filamentous fungi and its use to study control of nucleic acid synthesis in Aspergillus nidulans},
url = {http://dx.doi.org/10.1099/00221287-118-2-443},
volume = {118},
year = {1980}
}
TY - JOUR
AB - A method is described for measuring the syntheses of macromolecules in the filamentous fungus A. nidulans. It involves growth of mycelium on small filter papers in the presence of radioactively labelled L-leucine to monitor total protein synthesis, and hence growth, and following the incorporation of differently labelled D-uridine (to measure nucleic acid synthesis) or L-leucine (to measure protein synthesis) under various conditions such as starvation for a metabolite or the presence of an inhibitor. Comparison of <sup>3</sup>H/<sup>14</sup>C or <sup>14</sup>C/<sup>3</sup>H labelling ratios (depending on the labelling combination used) shows the effects of the treatments on nucleic acid or protein synthesis. The method allows large numbers of measurements to be made and enables economical use of radioisotopes. Results are presented to show that, under the labelling conditions used, uridine is incorporated mainly into stable RNA. The method is used to demonstrate probable stringent control of stable RNA synthesis in A. nidulans and that inhibitors of protein synthesis such as cycloheximide and anisomycin also inhibit stable RNA synthesis. In contrast, starvation for meso-inositol affects nucleic acid synthesis much more strongly than it affects protein synthesis.
AU - Arst,HN
AU - Scazzocchio,C
DO - 10.1099/00221287-118-2-443
EP - 454
PY - 1980///
SN - 0022-1287
SP - 443
TI - A method for monitoring macromolecular synthesis in filamentous fungi and its use to study control of nucleic acid synthesis in Aspergillus nidulans
T2 - Journal of General Microbiology
UR - http://dx.doi.org/10.1099/00221287-118-2-443
VL - 118
ER -
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