In this section
@article{Ledesma-Amaro:2023:10.1186/s12934-023-02184-1,
author = {Ledesma-Amaro, R and Sun, M and Ledesma, Amaro R and Xiong, Gao A and Liu, X and Nie, J and Bai, Z},
doi = {10.1186/s12934-023-02184-1},
journal = {Microbial Cell Factories},
title = {High-throughput process development from gene cloning to protein production},
url = {http://dx.doi.org/10.1186/s12934-023-02184-1},
volume = {22},
year = {2023}
}
TY - JOUR
AB - In the post-genomic era, the demand for faster and more efficient protein production has increased, both in public laboratories and industry. In addition, with the expansion of protein sequences in databases, the range of possible enzymes of interest for a given application is also increasing. Faced with peer competition, budgetary, and time constraints, companies and laboratories must find ways to develop a robust manufacturing process for recombinant protein production. In this review, we explore high-throughput technologies for recombinant protein expression and present a holistic high-throughput process development strategy that spans from genes to proteins. We discuss the challenges that come with this task, the limitations of previous studies, and future research directions.
AU - Ledesma-Amaro,R
AU - Sun,M
AU - Ledesma,Amaro R
AU - Xiong,Gao A
AU - Liu,X
AU - Nie,J
AU - Bai,Z
DO - 10.1186/s12934-023-02184-1
PY - 2023///
SN - 1475-2859
TI - High-throughput process development from gene cloning to protein production
T2 - Microbial Cell Factories
UR - http://dx.doi.org/10.1186/s12934-023-02184-1
UR - https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-023-02184-1
VL - 22
ER -
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