BibTex format
@article{Hewitt:2023:10.1038/s41467-023-41621-y.,
author = {Hewitt, R and Puttur, F and Gaboriau, D and Fercoq, F and Fresquet, M and Traves, W and Yates, LL and Walker, S and Molyneaux, P and Kemp, S and Nicholson, A and Rice, A and Roberts, E and Lennon, R and Carlin, L and Byrne, A and Maher, T and Lloyd, C},
doi = {10.1038/s41467-023-41621-y.},
journal = {Nature Communications},
title = {Lung extracellular matrix modulates KRT5+ basal cell activity in pulmonary fibrosis},
url = {http://dx.doi.org/10.1038/s41467-023-41621-y.},
volume = {14},
year = {2023}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Aberrant expansion of KRT5+ basal cells in the distal lung accompanies progressive alveolar epithelial cell loss and tissue remodelling during fibrogenesis in idiopathic pulmonary fibrosis (IPF). The mechanisms determining activity of KRT5+ cells in IPF have not been delineated. Here, we reveal a potential mechanism by which KRT5+ cells migrate within the fibrotic lung, navigating regional differences in collagen topography. In vitro, KRT5+ cell migratory characteristics and expression of remodelling genes are modulated by extracellular matrix (ECM) composition and organisation. Mass spectrometry- based proteomics revealed compositional differences in ECM components secreted by primary human lung fibroblasts (HLF) from IPF patients compared to controls. Over-expression of ECM glycoprotein, Secreted Protein Acidic and Cysteine Rich (SPARC) in the IPF HLF matrix restricts KRT5+ cell migration in vitro. Together, our findings demonstrate how changes to the ECM in IPF directly influence KRT5+ cell behaviour and function contributing to remodelling events in the fibrotic niche.
AU - Hewitt,R
AU - Puttur,F
AU - Gaboriau,D
AU - Fercoq,F
AU - Fresquet,M
AU - Traves,W
AU - Yates,LL
AU - Walker,S
AU - Molyneaux,P
AU - Kemp,S
AU - Nicholson,A
AU - Rice,A
AU - Roberts,E
AU - Lennon,R
AU - Carlin,L
AU - Byrne,A
AU - Maher,T
AU - Lloyd,C
DO - 10.1038/s41467-023-41621-y.
PY - 2023///
SN - 2041-1723
TI - Lung extracellular matrix modulates KRT5+ basal cell activity in pulmonary fibrosis
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-023-41621-y.
VL - 14
ER -