In this section
@article{Hassan:2023:10.1099/jgv.0.001925,
author = {Hassan, S and Syun-Ichi, U and Shabeer, S and Wu, C-F and Moriyama, H and Coutts, RHA and Kotta-Loizou, I and Jamal, A},
doi = {10.1099/jgv.0.001925},
journal = {Journal of General Virology},
title = {Molecular and biological characterization of a partitivirus from Paecilomyces variotii},
url = {http://dx.doi.org/10.1099/jgv.0.001925},
volume = {104},
year = {2023}
}
TY - JOUR
AB - Paeciliomyces variotii is a thermo-tolerant, ubiquitous fungus commonly found in food products, indoor environments, soil and clinical samples. It is a well-known biocontrol agent used against phytopathogenic fungi and its metabolites have many industrial applications. Rare reports of P. variotii-related human infections have been found in the medical literature. In this study, we report for the first time the infection of P. variotii isolated from a soil sample collected in a rice field with a double-stranded RNA virus, Paeciliomyces variotii partitivirus 1 (PvPV-1) in the family Partitiviridae. P. variotii harboured icosahedral virus particles 30 nm in diameter with two dsRNA segments 1758 and 1356 bp long. Both dsRNA1 and dsRNA2 have a single open reading frame encoding proteins of 63 and 40 kDa, respectively. These proteins have significant similarity to the RNA-dependent RNA polymerase and capsid protein encoded by the genomic segments of several viruses from the family Partitiviridae. Phylogenetic analysis revealed that PvPV-1 belongs to the family Partitiviridae but in an unclassified group/genus, tentatively nominated Zetapartitivirus. PvPV-1 was found to increase the growth rate of the host fungus, as indicated by time course experiments performed on a range of different media for virus-infected and virus-free isogenic lines. Further, dual-culture assays performed for both isogenic lines confirmed the antagonistic potential of P. variotii against other phytopathogenic fungi. The findings of this study assist us in understanding P. variotii as a potential biocontrol agent, together with plant-fungus-virus interactions.
AU - Hassan,S
AU - Syun-Ichi,U
AU - Shabeer,S
AU - Wu,C-F
AU - Moriyama,H
AU - Coutts,RHA
AU - Kotta-Loizou,I
AU - Jamal,A
DO - 10.1099/jgv.0.001925
PY - 2023///
SN - 0022-1317
TI - Molecular and biological characterization of a partitivirus from Paecilomyces variotii
T2 - Journal of General Virology
UR - http://dx.doi.org/10.1099/jgv.0.001925
UR - https://www.ncbi.nlm.nih.gov/pubmed/38015047
UR - https://doi.org/10.1099/jgv.0.001925
VL - 104
ER -
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