In this section
@article{Ehsan:2025:10.1002/cbic.202400958,
author = {Ehsan, M and Ghani, L and Lan, B and Katsube, S and Poulsen, IH and Zhang, X and Arslan, M and Byrne, B and Loland, CJ and Guan, L and Liu, X and Chae, PS},
doi = {10.1002/cbic.202400958},
journal = {ChemBioChem: a European journal of chemical biology},
title = {Unsymmetric triazinebased triglucoside detergents for membrane protein stability},
url = {http://dx.doi.org/10.1002/cbic.202400958},
volume = {26},
year = {2025}
}
TY - JOUR
AB - Membrane proteins play a crucial role in a variety of biological processes and are key targets for pharmaceutical development. Structural studies of membrane proteins provide molecular insights into the mechanisms of these processes and are essential for effective drug discovery. Historically, these studies have relied on solubilization of the target protein using detergents, but conventional detergents often fail to maintain the stability of challenging membrane proteins. To address this issue, there is a need to develop novel detergents with enhanced protein stabilization properties. In this study, we synthesized unsymmetric variants of recently reported tris(hydroxymethyl)aminomethane(TRIS)-linker-bearing triazine-based triglucosides (TTGs) by incorporating two different alkyl chains (long and short) into the detergent structure. When tested with model membrane proteins, including a G protein-coupled receptor, TTG-8,12 demonstrated superior efficacy in stabilizing membrane proteins compared to the original TTGs and the gold standard detergents DDM/LMNG. These results suggest that detergent unsymmetry is an important concept for improving detergent performance and unsymmetric detergents such as TTG-8,12 hold significant potential for advancing membrane protein structural studies.
AU - Ehsan,M
AU - Ghani,L
AU - Lan,B
AU - Katsube,S
AU - Poulsen,IH
AU - Zhang,X
AU - Arslan,M
AU - Byrne,B
AU - Loland,CJ
AU - Guan,L
AU - Liu,X
AU - Chae,PS
DO - 10.1002/cbic.202400958
PY - 2025///
SN - 1439-4227
TI - Unsymmetric triazinebased triglucoside detergents for membrane protein stability
T2 - ChemBioChem: a European journal of chemical biology
UR - http://dx.doi.org/10.1002/cbic.202400958
UR - https://doi.org/10.1002/cbic.202400958
VL - 26
ER -
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