BibTex format
@article{Sun:2025:10.1016/j.synbio.2025.08.001,
author = {Sun, M and Zhao, Y and Ledesma-Amaro, R and Gao, J and Liu, X and Bai, Z and Gao, AX and Wang, P},
doi = {10.1016/j.synbio.2025.08.001},
journal = {Synthetic and Systems Biotechnology},
pages = {1331--1340},
title = {Engineered membraneless organelles in Corynebacterium glutamicum for enhanced indigoidine biosynthesis and antimicrobial peptide production},
url = {http://dx.doi.org/10.1016/j.synbio.2025.08.001},
volume = {10},
year = {2025}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Liquid-liquid phase separation (LLPS)-driven membraneless organelles (MLOs) have been employed to enhance metabolic efficiency in various microbial cell factories. However, their application in the industrial bacterium Corynebacterium glutamicum has not been explored. Here, we report the formation of liquid protein condensates in C. glutamicum using the RGG domain of Caenorhabditis elegans LAF-1. We optimized conditions for condensate formation, including the pre-induction period, inducer concentration, and cultivation temperature. Using the indigoidine biosynthesis pathway as a model, we demonstrated that LLPS-mediated MLOs enhanced indigoidine production. Furthermore, we applied these MLOs to modulate the toxicity of antimicrobial peptides (AMPs) to host cells, facilitating the expression of AMPs, including melittin and lactoferricin B. These findings provide insights into MLOs engineering in C. glutamicum and suggest broader applications of LLPS-mediated systems in industrial biotechnology.
AU - Sun,M
AU - Zhao,Y
AU - Ledesma-Amaro,R
AU - Gao,J
AU - Liu,X
AU - Bai,Z
AU - Gao,AX
AU - Wang,P
DO - 10.1016/j.synbio.2025.08.001
EP - 1340
PY - 2025///
SN - 2405-805X
SP - 1331
TI - Engineered membraneless organelles in Corynebacterium glutamicum for enhanced indigoidine biosynthesis and antimicrobial peptide production
T2 - Synthetic and Systems Biotechnology
UR - http://dx.doi.org/10.1016/j.synbio.2025.08.001
UR - https://www.ncbi.nlm.nih.gov/pubmed/40893468
VL - 10
ER -