BibTex format
@article{Worland:2026:10.1016/j.tibtech.2025.08.019,
author = {Worland, AM and Xu, VA and Duran, MF and Gitman, P and Hunter-Cevera, K and Klemm, C and Sun, Y and Sanchis, DR and Ledesma-Amaro, R and Pomraning, KR and Tanjore, D and Blenner, M and Tang, YJ},
doi = {10.1016/j.tibtech.2025.08.019},
journal = {Trends Biotechnol},
pages = {154--169},
title = {Staying productive under pressure: systems evaluations of β-carotene production in Yarrowia lipolytica under continuous fermentation.},
url = {http://dx.doi.org/10.1016/j.tibtech.2025.08.019},
volume = {44},
year = {2026}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Scaling biomanufacturing from laboratory to industrial scale poses significant challenges, especially for continuous fermentation. This study investigates these challenges using a β-carotene-producing Yarrowia lipolytica strain. Through fermentation experiments and proteomics, we have assessed how fermentation modes, carbon sources, dissolved O2, and media composition influence long-term bioproduction. In shaking flask subcultures, the strain maintained β-carotene production for over ~30 generations. However, in continuous fermentations, subpopulation shifted toward faster-growing low-producers, leading to significant production losses within just ~18 growth generations. This process was accelerated by O2 limitation and high bioreactor dilution rates. Using canola oil as a carbon source increases population heterogeneity but enhances β-carotene biosynthesis and prolongs production compared with glucose-based media. Kinetic modeling suggests that strains optimized for the highest production in laboratory settings may be less robust in industrial environments, where suboptimal yet faster-growing variants gain a competitive edge under prolonged stress and ultimately shape overall continuous fermentation performance.
AU - Worland,AM
AU - Xu,VA
AU - Duran,MF
AU - Gitman,P
AU - Hunter-Cevera,K
AU - Klemm,C
AU - Sun,Y
AU - Sanchis,DR
AU - Ledesma-Amaro,R
AU - Pomraning,KR
AU - Tanjore,D
AU - Blenner,M
AU - Tang,YJ
DO - 10.1016/j.tibtech.2025.08.019
EP - 169
PY - 2026///
SP - 154
TI - Staying productive under pressure: systems evaluations of β-carotene production in Yarrowia lipolytica under continuous fermentation.
T2 - Trends Biotechnol
UR - http://dx.doi.org/10.1016/j.tibtech.2025.08.019
UR - https://www.ncbi.nlm.nih.gov/pubmed/40987704
VL - 44
ER -