In this section
@article{Collins:2025:10.1016/j.copbio.2025.103390,
author = {Collins, K and Stanley, CE and Ouldridge, TE},
doi = {10.1016/j.copbio.2025.103390},
journal = {Current Opinion in Biotechnology},
title = {Biochemical surface patterning in microfluidic devices},
url = {http://dx.doi.org/10.1016/j.copbio.2025.103390},
volume = {96},
year = {2025}
}
TY - JOUR
AB - The capacity to pattern biomolecules within microfluidic devices expands the scope of microfluidic technologies. In such patterned systems, surface-bound components remain localized, while the microfluidic network supplies reagents and removes waste products. This approach has enabled continuous protein expression from patterned DNA, chemical synthesis from immobilized enzymes, and cell capture assays. Here, we review methods to pattern surfaces within microfluidic devices. Patterns may be printed before or after the device is assembled; pre-bonding methods are compatible with well-established open-surface patterning protocols but present challenges for device bonding and alignment. Conversely, post-bonding methods are compatible with standard bonding procedures but rely on less established, sequential patterning protocols. Future progress will require consistent reporting of pattern signal and noise relative to controls.
AU - Collins,K
AU - Stanley,CE
AU - Ouldridge,TE
DO - 10.1016/j.copbio.2025.103390
PY - 2025///
SN - 0958-1669
TI - Biochemical surface patterning in microfluidic devices
T2 - Current Opinion in Biotechnology
UR - http://dx.doi.org/10.1016/j.copbio.2025.103390
UR - https://doi.org/10.1016/j.copbio.2025.103390
VL - 96
ER -
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