In this section
@article{Bailly:2005:10.1385/1-59259-823-4:319,
author = {Bailly, C and Kluza, J and Martin, C and Ellis, T and Waring, MJ},
doi = {10.1385/1-59259-823-4:319},
journal = {Methods Mol Biol},
pages = {319--342},
title = {DNase I footprinting of small molecule binding sites on DNA.},
url = {http://dx.doi.org/10.1385/1-59259-823-4:319},
volume = {288},
year = {2005}
}
TY - JOUR
AB - Nuclease footprinting techniques were initially developed to investigate protein-deoxyribonucleic acid (DNA) interactions but these tools of molecular biology have also become instrumental for probing sequence-selective binding of small molecules to DNA. Here, the method is described and technical details are given for performing deoxyribonuclease (DNase) I footprinting with DNA-binding drugs. An example is presented where DNase I is used (as well as DNase II and micrococcal nuclease) to probe the patterns of sequence-selective recognition of DNA by the anticancer antibiotic actinomycin D. DNase I is a convenient endonuclease for detecting and locating the position of actinomycin-binding sites within GC-rich sequences.
AU - Bailly,C
AU - Kluza,J
AU - Martin,C
AU - Ellis,T
AU - Waring,MJ
DO - 10.1385/1-59259-823-4:319
EP - 342
PY - 2005///
SN - 1064-3745
SP - 319
TI - DNase I footprinting of small molecule binding sites on DNA.
T2 - Methods Mol Biol
UR - http://dx.doi.org/10.1385/1-59259-823-4:319
UR - https://www.ncbi.nlm.nih.gov/pubmed/15333913
VL - 288
ER -
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