BibTex format
@misc{Ellis:2008,
author = {Ellis, T and Wang, X and Collins, JJ},
title = {Diversity-Based Design of Synthetic Gene Networks with Desired Functions},
type = {Poster},
year = {2008}
}
Citation
RIS format (EndNote, RefMan)
TY - GEN
AB - Constructing predictable gene networks with desired functions remains hampered by the lack of well-characterized components and the fact that assembled networks often require extensive, iterative retrofitting for optimization. Here we present an approach where network components are synthesized with random sequences incorporated into their design, giving rapid parallel production of component libraries with inherent diversity. When coupled with in silico modeling, libraries present a choice of characterized parts for gene network design, and those optimal for the desired function can be selected for network assembly, without the need for post-hoc tweaking. We validated our approach in yeast (S.cerevisiae) by synthesizing a regulatory promoter library and using it to construct negative feedforward loop networks with different, desired input-output characteristics. We then implemented the method to produce a synthetic gene network that acts as a timer, tunable by component choice. We utilize this network to control the timing of the yeast flocculation phenotype, which is crucial to brewing, illustrating a practical application of our approach.
AU - Ellis,T
AU - Wang,X
AU - Collins,JJ
PY - 2008///
TI - Diversity-Based Design of Synthetic Gene Networks with Desired Functions
ER -
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