In this section
@article{Findon:2010:10.1016/j.fgb.2010.04.002,
author = {Findon, H and Calcagno-Pizarelli, AM and Martínez, JL and Spielvogel, A and Markina-Iñarrairaegui, A and Indrakumar, T and Ramos, J and Peñalva, MA and Espeso, EA and Arst, HN},
doi = {10.1016/j.fgb.2010.04.002},
journal = {Fungal Genet Biol},
pages = {647--655},
title = {Analysis of a novel calcium auxotrophy in Aspergillus nidulans.},
url = {http://dx.doi.org/10.1016/j.fgb.2010.04.002},
volume = {47},
year = {2010}
}
TY - JOUR
AB - In Aspergillus nidulans a combination of null mutations in halA, encoding a protein kinase, and sltA, encoding a zinc-finger transcription factor having no yeast homologues, results in an elevated calcium requirement ('calcium auxotrophy') without impairing net calcium uptake. sltA(-) (+/-halA(-)) mutations result in hypertrophy of the vacuolar system. In halA(-)sltA(-) (and sltA(-)) strains, transcript levels for pmcA and pmcB, encoding vacuolar Ca(2+)-ATPase homologues, are highly elevated, suggesting a regulatory relationship between vacuolar membrane area and certain vacuolar membrane ATPase levels. Deletion of both pmcA and pmcB strongly suppresses the 'calcium auxotrophy'. Therefore the 'calcium auxotrophy' possibly results from excessive vacuolar calcium sequestration, causing cytosolic calcium deprivation. Null mutations in nhaA, homologous to Saccharomyces cerevisiae NHA1, encoding a plasma membrane Na(+)/H(+) antiporter effluxing Na(+) and K(+), and a non-null mutation in trkB, homologous to S. cerevisiae TRK1, encoding a plasma membrane high affinity K(+) transporter, also suppress the calcium auxotrophy.
AU - Findon,H
AU - Calcagno-Pizarelli,AM
AU - Martínez,JL
AU - Spielvogel,A
AU - Markina-Iñarrairaegui,A
AU - Indrakumar,T
AU - Ramos,J
AU - Peñalva,MA
AU - Espeso,EA
AU - Arst,HN
DO - 10.1016/j.fgb.2010.04.002
EP - 655
PY - 2010///
SN - 1096-0937
SP - 647
TI - Analysis of a novel calcium auxotrophy in Aspergillus nidulans.
T2 - Fungal Genet Biol
UR - http://dx.doi.org/10.1016/j.fgb.2010.04.002
VL - 47
ER -
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