In this section
@article{Blount:2018:10.1038/s41467-018-03143-w,
author = {Blount, B and Gowers, G and Ho, JCH and Ledesma-Amaro, R and Jovicevic, D and McKiernan, R and Xie, ZX and Li, BZ and Yuan, YJ and Ellis, T},
doi = {10.1038/s41467-018-03143-w},
journal = {Nature Communications},
title = {Rapid host strain improvement by in vivo rearrangement of a synthetic yeast chromosome},
url = {http://dx.doi.org/10.1038/s41467-018-03143-w},
volume = {9},
year = {2018}
}
TY - JOUR
AB - Synthetic biology tools, such as modular parts and combinatorial DNA assembly, are routinely used to optimise the productivity of heterologous metabolic pathways for biosynthesis or substrate utilisation, yet, it is well established that host strain background is just as important for determining productivity. Here we report that in vivo combinatorial genomic rearrangement of Saccharomyces cerevisiae yeast with a synthetic chromosome V can rapidly generate new, improved host strains with genetic backgrounds favourable to diverse heterologous pathways, including those for violacein and penicillin biosynthesis and for xylose utilisation. We show how the modular rearrangement of synthetic chromosomes by SCRaMbLE can be easily determined using long-read nanopore sequencing and we explore experimental conditions that optimise diversification and screening. This new synthetic genome approach to metabolic engineering provides productivity improvements in a fast, simple and accessible way, making it a valuable addition to existing strain improvement techniques.
AU - Blount,B
AU - Gowers,G
AU - Ho,JCH
AU - Ledesma-Amaro,R
AU - Jovicevic,D
AU - McKiernan,R
AU - Xie,ZX
AU - Li,BZ
AU - Yuan,YJ
AU - Ellis,T
DO - 10.1038/s41467-018-03143-w
PY - 2018///
SN - 2041-1723
TI - Rapid host strain improvement by in vivo rearrangement of a synthetic yeast chromosome
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-018-03143-w
UR - https://www.nature.com/articles/s41467-018-03143-w
VL - 9
ER -
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