BibTex format
@article{Andersson:2020:10.12688/wellcomeopenres.16002.2,
author = {Andersson, MI and Arancibia-Carcamo, CV and Auckland, K and Baillie, JK and Barnes, E and Beneke, T and Bibi, S and Brooks, T and Carroll, M and Crook, D and Dingle, K and Dold, C and Downs, LO and Dunn, L and Eyre, DW and Gilbert, Jaramillo J and Harvala, H and Hoosdally, S and Ijaz, S and James, T and James, W and Jeffery, K and Justice, A and Klenerman, P and Knight, JC and Knight, M and Liu, X and Lumley, SF and Matthews, PC and McNaughton, AL and Mentzer, AJ and Mongkolsapaya, J and Oakley, S and Oliveira, MS and Peto, T and Ploeg, RJ and Ratcliff, J and Robbins, MJ and Roberts, DJ and Rudkin, J and Russell, RA and Screaton, G and Semple, MG and Skelly, D and Simmonds, P and Stoesser, N and Turtle, L and Wareing, S and Zambon, M},
doi = {10.12688/wellcomeopenres.16002.2},
journal = {Wellcome Open Res},
title = {SARS-CoV-2 RNA detected in blood products from patients with COVID-19 is not associated with infectious virus.},
url = {http://dx.doi.org/10.12688/wellcomeopenres.16002.2},
volume = {5},
year = {2020}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Background: Laboratory diagnosis of SARS-CoV-2 infection (the cause of COVID-19) uses PCR to detect viral RNA (vRNA) in respiratory samples. SARS-CoV-2 RNA has also been detected in other sample types, but there is limited understanding of the clinical or laboratory significance of its detection in blood. Methods: We undertook a systematic literature review to assimilate the evidence for the frequency of vRNA in blood, and to identify associated clinical characteristics. We performed RT-PCR in serum samples from a UK clinical cohort of acute and convalescent COVID-19 cases (n=212), together with convalescent plasma samples collected by NHS Blood and Transplant (NHSBT) (n=462 additional samples). To determine whether PCR-positive blood samples could pose an infection risk, we attempted virus isolation from a subset of RNA-positive samples. Results: We identified 28 relevant studies, reporting SARS-CoV-2 RNA in 0-76% of blood samples; pooled estimate 10% (95%CI 5-18%). Among serum samples from our clinical cohort, 27/212 (12.7%) had SARS-CoV-2 RNA detected by RT-PCR. RNA detection occurred in samples up to day 20 post symptom onset, and was associated with more severe disease (multivariable odds ratio 7.5). Across all samples collected ≥28 days post symptom onset, 0/494 (0%, 95%CI 0-0.7%) had vRNA detected. Among our PCR-positive samples, cycle threshold (ct) values were high (range 33.5-44.8), suggesting low vRNA copy numbers. PCR-positive sera inoculated into cell culture did not produce any cytopathic effect or yield an increase in detectable SARS-CoV-2 RNA. There was a relationship between RT-PCR negativity and the presence of total SARS-CoV-2 antibody (p=0.02). Conclusions: vRNA was detectable at low viral loads in a minority of serum samples collected in acute infection, but was not associated with infectious SARS-CoV-2 (within the limitations of the assays used). This work helps to inform biosafety precautions for handling blood products from patients with c
AU - Andersson,MI
AU - Arancibia-Carcamo,CV
AU - Auckland,K
AU - Baillie,JK
AU - Barnes,E
AU - Beneke,T
AU - Bibi,S
AU - Brooks,T
AU - Carroll,M
AU - Crook,D
AU - Dingle,K
AU - Dold,C
AU - Downs,LO
AU - Dunn,L
AU - Eyre,DW
AU - Gilbert,Jaramillo J
AU - Harvala,H
AU - Hoosdally,S
AU - Ijaz,S
AU - James,T
AU - James,W
AU - Jeffery,K
AU - Justice,A
AU - Klenerman,P
AU - Knight,JC
AU - Knight,M
AU - Liu,X
AU - Lumley,SF
AU - Matthews,PC
AU - McNaughton,AL
AU - Mentzer,AJ
AU - Mongkolsapaya,J
AU - Oakley,S
AU - Oliveira,MS
AU - Peto,T
AU - Ploeg,RJ
AU - Ratcliff,J
AU - Robbins,MJ
AU - Roberts,DJ
AU - Rudkin,J
AU - Russell,RA
AU - Screaton,G
AU - Semple,MG
AU - Skelly,D
AU - Simmonds,P
AU - Stoesser,N
AU - Turtle,L
AU - Wareing,S
AU - Zambon,M
DO - 10.12688/wellcomeopenres.16002.2
PY - 2020///
SN - 2398-502X
TI - SARS-CoV-2 RNA detected in blood products from patients with COVID-19 is not associated with infectious virus.
T2 - Wellcome Open Res
UR - http://dx.doi.org/10.12688/wellcomeopenres.16002.2
UR - https://www.ncbi.nlm.nih.gov/pubmed/33283055
VL - 5
ER -
