In this section
@inproceedings{Mickleburgh:2020,
author = {Mickleburgh, TG and Salehi-Reyhani, A and Magness, AJ and Joyce, WD and Ces, O and Klug, DR},
pages = {183--184},
title = {A miniaturized microfluidic assay for single plant cell protein quantitation},
year = {2020}
}
TY - CPAPER
AB - We present a method based on a miniaturized antibody capture chip to perform protein quantitation in plants with single cell single molecule resolution. We demonstrate the versatility of the method in capturing and analyzing membrane, cytosolic, and chloroplast stroma located proteins, specifically demonstrated by SNARE 12: YFP, 35S: GFP, and Ribulose-1, 5-bisphosphate carboxylase/oxygenase, respectively. Single cell protein expression quantification is achieved through a bi or tri-molecular antibody microarray. We have also reported single cell experimental evidence that a reduction in chamber volume enhances assay sensitivity.
AU - Mickleburgh,TG
AU - Salehi-Reyhani,A
AU - Magness,AJ
AU - Joyce,WD
AU - Ces,O
AU - Klug,DR
EP - 184
PY - 2020///
SP - 183
TI - A miniaturized microfluidic assay for single plant cell protein quantitation
ER -
For more information, please contact one of the Group Leaders: