Protein Production and Analysis (PPA) Facility

The TECAN Freedom EVO 200 Liquid Handling Robot increases throughput and brings improved efficiency to protein production and analysis.

The new state-of-the-art PPA Facility, located at Imperial’s South Kensington campus, enables high throughput production and analysis of proteins at atomic and cellular levels. In order to better understand the molecular mechanisms underlying bacterial pathogenesis and host resistance, there is an increasing need to identify, characterise and produce the key protein complexes involved. This understanding is crucial to the design of effective vaccines and antibacterial drugs.

The Tecan Freedom EVO 200 Liquid Handling Robot can be tailored for a variety of activities including accelerated gene cloning, protein expression and purification in both prokaryotic and eukaryotic host systems. The PPA equipment will enable the structural and molecular characterisation of important toxins and other virulence proteins and their complexes. It will also support the accelerated synthesis of protein reagents in sufficient quantities and purity for the subsequent mapping of protein-protein interaction networks. Such activities underpin atomic resolution studies, cellular assays, antibody production molecular screening and drug discovery.

Further information on the Facility can be found on the Centre for Molecular Bacteriology & Infection facilities website.