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Citation

BibTex format

@article{Dvinskikh:2025:10.1364/BOE.572592,
author = {Dvinskikh, L and Sparks, H and Rowe-Brown, L and Hong, W and Ennis, D and Sarnataro, R and Carreno, Yugueros D and Tissot, F and Lo, Celso C and Riglar, D and Cunnea, P and McNeish, I and Dunsby, C},
doi = {10.1364/BOE.572592},
journal = {Biomedical Optics Express},
pages = {3725--3739},
title = {Cleared tissue dual-view oblique plane microscopy},
url = {http://dx.doi.org/10.1364/BOE.572592},
volume = {16},
year = {2025}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - We present a dual-view oblique plane microscope (dOPM) for imaging thick optically cleared tissue samples using a silicone immersion primary objective. The custom-designed remote refocusing relay utilizes stock optics to achieve remote refocusing in refractive index-matched samples. The spatial resolution of the system was characterized using a series of fluorescent bead phantoms with refractive indices ranging from 1.4-1.5, with the point spread function full width at half maximum measuring ∼0.5 µm laterally and ∼1 µm axially for a refractive index-matched bead sample, with minimal degradation over a > 250 µm axial range. We characterize how the remote refocusing performance at sample refractive indices up to n = 1.5 can be partially compensated for using adjustment of the correction collar on the primary objective. We apply the system to imaging a range of biological samples with varied refractive indices. Combined with tiled acquisition, image stitching, and multi-view image fusion, the microscope enables multicolour imaging of mm-wide and ∼250 µm thick optically cleared mouse ovarian cancer, bone marrow, and colon tissue samples, and a whole Drosophila melanogaster fruit fly brain. The system offers a platform for fast and high-resolution, multicolour volumetric imaging across spatial scales, integrated on a commercially available microscope frame.
AU  - Dvinskikh,L
AU  - Sparks,H
AU  - Rowe-Brown,L
AU  - Hong,W
AU  - Ennis,D
AU  - Sarnataro,R
AU  - Carreno,Yugueros D
AU  - Tissot,F
AU  - Lo,Celso C
AU  - Riglar,D
AU  - Cunnea,P
AU  - McNeish,I
AU  - Dunsby,C
DO  - 10.1364/BOE.572592
EP  - 3739
PY  - 2025///
SN  - 2156-7085
SP  - 3725
TI  - Cleared tissue dual-view oblique plane microscopy
T2  - Biomedical Optics Express
UR  - http://dx.doi.org/10.1364/BOE.572592
VL  - 16
ER  -
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