Citation

BibTex format

@article{Ren:2023:10.1038/S41467-023-43004-9,
author = {Ren, R and Cai, S and Fang, X and Wang, X and Zhang, Z and Damiani, M and Hudlerova, C and Rosa, A and Hope, J and Cook, NJ and Gorelkin, P and Erofeev, A and Novak, P and Badhan, A and Crone, M and Freemont, P and Taylor, GP and Tang, L and Edwards, C and Shevchuk, A and Cherepanov, P and Luo, Z and Tan, W and Korchev, Y and Ivanov, AP and Edel, JB},
doi = {10.1038/S41467-023-43004-9},
journal = {Nature Communications},
title = {Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes},
url = {http://dx.doi.org/10.1038/S41467-023-43004-9},
volume = {14},
year = {2023}
}
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TY  - JOUR
AB  - We report on single-molecule nanopore sensing combined with position-encoded DNA molecular probes, with chemistry tuned to simultaneously identify various antigen proteins and multiple RNA gene fragments of SARS-CoV-2 with high sensitivity and selectivity. We show that this sensing strategy can directly detect spike (S) and nucleocapsid (N) proteins in unprocessed human saliva. Moreover, our approach enables the identification of RNA fragments from patient samples using nasal/throat swabs, enabling the identification of critical mutations such as D614G, G446S, or Y144del among viral variants. In particular, it can detect and discriminate between SARS-CoV-2 lineages of wild-type B.1.1.7 (Alpha), B.1.617.2 (Delta), and B.1.1.539 (Omicron) within a single measurement without the need for nucleic acid sequencing. The sensing strategy of the molecular probes is easily adaptable to other viral targets and diseases and can be expanded depending on the application required.
AU  - Ren,R
AU  - Cai,S
AU  - Fang,X
AU  - Wang,X
AU  - Zhang,Z
AU  - Damiani,M
AU  - Hudlerova,C
AU  - Rosa,A
AU  - Hope,J
AU  - Cook,NJ
AU  - Gorelkin,P
AU  - Erofeev,A
AU  - Novak,P
AU  - Badhan,A
AU  - Crone,M
AU  - Freemont,P
AU  - Taylor,GP
AU  - Tang,L
AU  - Edwards,C
AU  - Shevchuk,A
AU  - Cherepanov,P
AU  - Luo,Z
AU  - Tan,W
AU  - Korchev,Y
AU  - Ivanov,AP
AU  - Edel,JB
DO  - 10.1038/S41467-023-43004-9
PY  - 2023///
SN  - 2041-1723
TI  - Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/S41467-023-43004-9
UR  - https://www.nature.com/articles/s41467-023-43004-9
VL  - 14
ER  -
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