In this section
@article{Gall:2013:10.1186/1742-4690-10-8,
author = {Gall, A and Kaye, S and Hue, S and Bonsall, D and Rance, R and Baillie, GJ and Fidler, SJ and Weber, JN and McClure, MO and Kellam, P},
doi = {10.1186/1742-4690-10-8},
journal = {Retrovirology},
title = {Restriction of V3 region sequence divergence in the HIV-1 envelope gene during antiretroviral treatment in a cohort of recent seroconverters},
url = {http://dx.doi.org/10.1186/1742-4690-10-8},
volume = {10},
year = {2013}
}
TY - JOUR
AB - Background: Dynamic changes in Human Immunodeficiency Virus 1 (HIV-1) sequence diversity and divergence areassociated with immune control during primary infection and progression to AIDS. Consensus sequencing or singlegenome amplification sequencing of the HIV-1 envelope (env) gene, in particular the variable (V) regions, is used asa marker for HIV-1 genome diversity, but population diversity is only minimally, or semi-quantitatively sampledusing these methods.Results: Here we use second generation deep sequencing to determine inter-and intra-patient sequenceheterogeneity and to quantify minor variants in a cohort of individuals either receiving or not receivingantiretroviral treatment following seroconversion; the SPARTAC trial. We show, through a cross-sectional study ofsequence diversity of the env V3 in 30 antiretroviral-naive patients during primary infection that considerablepopulation structure diversity exists, with some individuals exhibiting highly constrained plasma virus diversity.Diversity was independent of clinical markers (viral load, time from seroconversion, CD4 cell count) of infection.Serial sampling over 60 weeks of non-treated individuals that define three initially different diversity profiles showedthat complex patterns of continuing HIV-1 sequence diversification and divergence could be readily detected.Evidence for minor sequence turnover, emergence of new variants and re-emergence of archived variants could beinferred from this analysis. Analysis of viral divergence over the same time period in patients who received short(12 weeks, ART12) or long course antiretroviral therapy (48 weeks, ART48) and a non-treated control group revealedthat ART48 successfully suppressed viral divergence while ART12 did not have a significant effect.Conclusions: Deep sequencing is a sensitive and reliable method for investigating the diversity of the env V3 as animportant component of HIV-1 genome diversity. Detailed insights into the complex early intra-patie
AU - Gall,A
AU - Kaye,S
AU - Hue,S
AU - Bonsall,D
AU - Rance,R
AU - Baillie,GJ
AU - Fidler,SJ
AU - Weber,JN
AU - McClure,MO
AU - Kellam,P
DO - 10.1186/1742-4690-10-8
PY - 2013///
SN - 1742-4690
TI - Restriction of V3 region sequence divergence in the HIV-1 envelope gene during antiretroviral treatment in a cohort of recent seroconverters
T2 - Retrovirology
UR - http://dx.doi.org/10.1186/1742-4690-10-8
UR - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000317052300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
VL - 10
ER -