In this section
@article{Demontis:2015:10.1002/jmv.24264,
author = {Demontis, MA and Sadiq, MT and Golz, S and Taylor, GP},
doi = {10.1002/jmv.24264},
journal = {Journal of Medical Virology},
pages = {4080--4101},
title = {HTLV-1 viral RNA is detected rarely in plasma of HTLV-1 infected subjects.},
url = {http://dx.doi.org/10.1002/jmv.24264},
volume = {120},
year = {2015}
}
TY - JOUR
AB - Plasma of patients infected with HTLV-1 is considered non-infectious but detection of HTLV-1 genomic RNA in plasma has been recently reported. The aim of this project was to detect and quantify HTLV-1 RNA in plasma and assess its potential value in diagnosis and prognosis. RNA from 1 ml of plasma from 65 subjects infected with HTLV-1 (27 asymptomatic carriers [AC]), 17 patients with HTLV-1-associated myelopathy (HAM/TSP), 14 with adult T-cell leukemia/lymphoma (ATLL), two co-infected with HIV, and five with other HTLV-1-associated disease, was extracted and reverse transcribed. HTLV-1 specific nested PCR was performed using primers to amplify the conserved Tax region. All samples were run in quadruplicate, nested PCR products were detected by gel electrophoresis. HTLV-1 RNA was detected in plasma from 18 (28%) patients, always at a very low copy number (3-13 copies viral cDNA per milliliter of plasma). Mean values of HTLV-1 proviral load did not differ between patients in whom HTLV-1 RNA was detected and patients in whom it was not possible to detect HTLV-1 RNA in plasma. HTLV-1 genomic RNA can be detected in the plasma of a minority of patients but not at a level or frequency to be useful clinically or diagnostically. Lack of transmission of HTLV-1 by plasma is due to the rare presence of HTLV-1 virions, regardless of any other factor. J. Med. Virol. © 2015 Wiley Periodicals, Inc.
AU - Demontis,MA
AU - Sadiq,MT
AU - Golz,S
AU - Taylor,GP
DO - 10.1002/jmv.24264
EP - 4101
PY - 2015///
SN - 1096-9071
SP - 4080
TI - HTLV-1 viral RNA is detected rarely in plasma of HTLV-1 infected subjects.
T2 - Journal of Medical Virology
UR - http://dx.doi.org/10.1002/jmv.24264
VL - 120
ER -