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Citation

BibTex format

@article{Lim:2019:10.1016/j.bmc.2019.06.025,
author = {Lim, C and Ha, KP and Clarke, R and Gavin, L-A and Cook, D and Hutton, J and Sutherell, C and Edwards, A and Evans, L and Tate, E and Lanyon-Hogg, T},
doi = {10.1016/j.bmc.2019.06.025},
journal = {Bioorganic and Medicinal Chemistry},
pages = {1--7},
title = {Identification of a potent small-molecule inhibitor of bacterial DNA repair that potentiates quinolone antibiotic activity in methicillin-resistant Staphylococcus aureus},
url = {http://dx.doi.org/10.1016/j.bmc.2019.06.025},
volume = {27},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The global emergence of antibiotic resistance is one of the most serious challenges facing modern medicine. There is an urgent need for validation of new drug targets and the development of small molecules with novel mechanisms of action. We therefore sought to inhibit bacterial DNA repair mediated by the AddAB/RecBCD protein complexes as a means to sensitize bacteria to DNA damage caused by the host immune system or quinolone antibiotics. A rational, hypothesis-driven compound optimization identified IMP-1700 as a cell-active, nanomolar potency compound. IMP-1700 sensitized multidrug-resistant Staphylococcus aureus to the fluoroquinolone antibiotic ciprofloxacin, where resistance results from a point mutation in the fluoroquinolone target, DNA gyrase. Cellular reporter assays indicated IMP-1700 inhibited the bacterial SOS-response to DNA damage, and compound-functionalized Sepharose successfully pulled-down the AddAB repair complex. This work provides validation of bacterial DNA repair as a novel therapeutic target and delivers IMP-1700 as a tool molecule and starting point for therapeutic development to address the pressing challenge of antibiotic resistance.
AU  - Lim,C
AU  - Ha,KP
AU  - Clarke,R
AU  - Gavin,L-A
AU  - Cook,D
AU  - Hutton,J
AU  - Sutherell,C
AU  - Edwards,A
AU  - Evans,L
AU  - Tate,E
AU  - Lanyon-Hogg,T
DO  - 10.1016/j.bmc.2019.06.025
EP  - 7
PY  - 2019///
SN  - 0968-0896
SP  - 1
TI  - Identification of a potent small-molecule inhibitor of bacterial DNA repair that potentiates quinolone antibiotic activity in methicillin-resistant Staphylococcus aureus
T2  - Bioorganic and Medicinal Chemistry
UR  - http://dx.doi.org/10.1016/j.bmc.2019.06.025
UR  - https://www.sciencedirect.com/science/article/pii/S096808961930536X
UR  - http://hdl.handle.net/10044/1/71612
VL  - 27
ER  -
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Contact

Prof. Ed Tate
GSK Chair in Chemical Biology
Department of Chemistry
Molecular Sciences Research Hub, White City Campus,
82 Wood Lane, London, W12 0BZ

e.tate@imperial.ac.uk
Tel: +44 (0)20 759 + ext 43752 or 45821