@article{Coulon:2010:10.1371/journal.pone.0014220,
author = {Coulon, C and Vinogradov, E and Filloux, A and Sadovskaya, I},
doi = {10.1371/journal.pone.0014220},
journal = {PLoS ONE},
title = {Chemical analysis of cellular and extracellular carbohydrates of a biofilm-forming strain pseudomonas aeruginosa PA14},
url = {http://dx.doi.org/10.1371/journal.pone.0014220},
volume = {5},
year = {2010}
}

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TY  - JOUR
AB  - BackgroundPseudomonas aeruginosa is a Gram-negative bacterium and an opportunistic pathogen, which causes persisting life-threatening infections in cystic fibrosis (CF) patients. Biofilm mode of growth facilitates its survival in a variety of environments. Most P. aeruginosa isolates, including the non-mucoid laboratory strain PA14, are able to form a thick pellicle, which results in a surface-associated biofilm at the air-liquid (A–L) interface in standing liquid cultures. Exopolysaccharides (EPS) are considered as key components in the formation of this biofilm pellicle. In the non-mucoid P. aeruginosa strain PA14, the “scaffolding” polysaccharides of the biofilm matrix, and the molecules responsible for the structural integrity of rigid A–L biofilm have not been identified. Moreover, the role of LPS in this process is unclear, and the chemical structure of the LPS O-antigen of PA14 has not yet been elucidated.Principal FindingsIn the present work we carried out a systematic analysis of cellular and extracellular (EC) carbohydrates of P. aeruginosa PA14. We also elucidated the chemical structure of the LPS O-antigen by chemical methods and 2-D NMR spectroscopy. Our results showed that it is composed of linear trisaccharide repeating units, identical to those described for P. aeruginosa Lanýi type O:2a,c (Lanýi-Bergman O-serogroup 10a, 10c; IATS serotype 19) and having the following structure: -4)-α-L-GalNAcA-(1–3)-α-D-QuiNAc-(1–3)- α-L-Rha-(1-. Furthermore, an EC O-antigen polysaccharide (EC O-PS) and the glycerol-phosphorylated cyclic β-(1,3)-glucans were identified in the culture supernatant of PA14, grown statically in minimal medium. Finally, the extracellular matrix of the thick biofilm formed at the A-L interface contained, in addition to eDNA, important quantities (at least ∼20% of dry weight) of LPS-like material.ConclusionsWe characterized the chemical structure of the LPS O-anti
AU  - Coulon,C
AU  - Vinogradov,E
AU  - Filloux,A
AU  - Sadovskaya,I
DO  - 10.1371/journal.pone.0014220
PY  - 2010///
SN  - 1932-6203
TI  - Chemical analysis of cellular and extracellular carbohydrates of a biofilm-forming strain pseudomonas aeruginosa PA14
T2  - PLoS ONE
UR  - http://dx.doi.org/10.1371/journal.pone.0014220
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000284939600013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/65719
VL  - 5
ER  - 

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