Hands-on LC-MS for Metabolic Profiling

Schedule

General Information

Day 1

Introductory lectures in mass spectrometry and chromatography, study design and sample preparation, followed by preparation of biological samples for analysis on subsequent days.

DAY 1 
TimeTopic
9.00- 9.30 Registration / Coffee
9.30- 10.45 Aims of course
  LC-MS for Metabolic Profiling
10.45-11.15 Break
11.15- 12.30 Sample Prep - untargeted
12.30-13.15 Lunch
13.15- 14.30 Untargeted analysis sample preparation
14.30-15.00 Break
15.00-16.30 Targeted analysis sample preparation
16.30-16.45 Summary/Debrief session
17.00-19.00 Welcome Mixer
 
Summary of the table's contents

Day 2

Analysis of biofluids through global profiling and targeted analyses; an introductory session to liquid chromatography, followed by sessions spent on each of the newest QToF instrumentation and the newest TQ instrumentation. Instrument set up, method development and acquisition will be covered. As we have set a maximum of 3 attendees per instrument this allows for hands-on participation by all. Day 3 finishes with introduction to data analysis.

DAY 2 
TimeTopic
 9.00 -9.30  Coffee Break
 9.30- 10.45  LC Session 1
 10.45-11.15  Break
 11.15- 12.30  LC Session 1
 12.30-13.15  Lunch
 13.15 -14.30  LC Session 1
 14.30-15.00  Break
 15.00-16.30  LC Session 1
 16.30-16.45  Summary/Debrief Session
 18.00  Course Dinner
 
Summary of the table's contents

Day 3

Analysis of biofluids through global profiling and targeted analyses; an introductory session to liquid chromatography, followed by sessions spent on each of the newest QToF instrumentation and the newest TQ instrumentation. Instrument set up, method development and acquisition will be covered. As we have set a maximum of 3 attendees per instrument this allows for hands-on participation by all. Day 3 finishes with introduction to data analysis.

 
DAY 3 
TimeTopic
 9.00 -9.30  Coffee Break
 9.30- 10.45  MC Session 2
 10.45-11.15  Break
 11.15- 12.30  MC Session 2
 12.30-13.15  Lunch
 13.15 -14.30 Introduction to Statistics
 14.30-15.00  Break
 15.00-16.30  Further Statistics: OPLS
 
Summary of the table's contents

Day 4

Data analysis workshops where attendees will process the data acquired from the previous days, mixed with further statistics lectures, allowing for development of interpretation skills.

 
DAY 4 
TimeTopic
 9.00-9.30  Coffee Break
 9.30- 10.45  Untargeted Data Analysis
 10.45-11.15  Break
 11.15- 12.30  Untargeted Data Analysis
 12.30-13.15  Lunch
 13.15 -14.30  Metabolite ID
 14.30-15.00  Break
 15.00-16.30  Targeted Data Analysis
 16.30-16.45  Debrief
Summary of the table's contents

Day 5

Application lectures, tips, tricks and troubleshooting.

Day 5 
TimeTopic
9.00- 9.30 Coffee Break
9.30- 10.45 Problem Solving Session
10.45-11.15 Break
11.15- 12.30 Problem Solving Session
12.30-13.15 Lunch
13.15- 14.30 Tips and Tricks for Metabolic Profiling
14.30-15.00 Break
15.00-16.30 Optional trip: National Centre