Debora Olivier is a cell biologist by training with a strong interest in advanced microscopies. She is currently a research engineer in Laure Crabbé’s lab (now at CBI Toulouse) and developed both imaging and analysis approaches to study telomere dynamics and spatial organisation. 

During her seminar, she will present a pipeline combining high-resolution 3D imaging of telomeres and nuclear envelope proteins at specific cell cycle stages, complemented with novel 3D spatial statistics and modelling. Using this approach, she will demonstrate a non-random organisation of telomeres in the nucleus, and a dynamic repositioning from the periphery to the interior as cells progress through the cell cycle. Time permitting, she will also present a tool to go from live-imaging movies to figures. This tool combines CellProfiler and Jupyter Notebooks to Segmentat, track and temporally align them to a reference time and generate graphs for multiple parameters.