The Workshop will provide participants with all the necessary knowledge and practical skill to implement this technique in their own laboratories.
Living Myocardial Slices (LMS) are a novel preparation of intermediate complexity that can be used to study heart biology. LMS possess complex 3D structure, heterocellularity and intact extracellular matrix that makes them ideal for functional and structural studies.LMS can be prepared from several species, including human, Multiple slices can be produced from each tissue or biopsy, producing several samples for experimentation (Watson et al, Nature Protocols, 2017;Watson et al. Nature Comms 2019; Pitoulis et al, Cardiovasc Res, 2021). LMS are highly viable, stable in culture and can be used for toxicology and pharmacology experiments, to study pathophysiology and treatments for cardiac disease.
While LMS are prepared by a straightforward method -simple mechanical dissociation using a vibrating microtome – several essential details contribute to the quality and viability, and this explains the variability of results in different laboratories. This workshop will discuss LMS in the context of other myocardial in vitro models, highlighting strengths and limitations of the technique, demonstrating the preparation of this model and offering the participants a hands-on opportunity to learn this technique.
Fee: £225 Academic, £450 Industry.
Deadline: 8th June 2022. Contact d.chokron20@imperial.ac.uk for more information