Dear FILM users and Imperial College London research staff,
Abberior and Photon Lines are delighted to invite you to a demonstration of our groundbreaking MINFLUX 3D nanoscope. Hosted by Alejandra Tomas (Department of Metabolism, Digestion and Reproduction) and Volodymyr Nechyporuk-Zloy (FILM) at ICTEM Building, this event will take place between the 12th and 16th of May.
Our MINFLUX nanoscope integrates many of the standard features of confocal microscopy – multiple excitation lines (e.g. 405 nm, 488 nm, 561 nm, and MINFLUX capable 640 nm) and dedicated detection channels (such as those for DAPI, GFP, Cy3, and Cy5) – with a confocal beam scanner offering an expansive field of view. Moreover, a sophisticated yet user-friendly graphical interface provides predefined imaging workflows that streamline the process.
At the heart of the instrument is a high-performance MINFLUX imaging mode, enabling you to resolve structures down to the molecular level across three dimensions. By probing for the location of the emitter with an excitation minimum, routine 3D MINFLUX imaging achieves isotropic localization precisions below 3 nm. The system also supports simultaneous two-colour MINFLUX imaging; a ratiometric approach for two-colour imaging is made possible by utilising dyes that, while excited at the same wavelength, emit at different wavelengths. Typically, a dye with an emission maximum around 665 nm (for example, Alexa Fluor 647) is paired with a red-shifted dye (such as CF680, with an emission maximum near 700 nm). This innovative multi-colour imaging capability in the nanometer range offers scientists an ideal tool to address a wide array of biomedical and biophysical questions at the molecular scale. In addition to nanoscale imaging, the MINFLUX microscope offers exceptional spatio-temporal resolution when used in its ‘Tracking’ modes, with sampling frequencies of up to 10 kHz. The Abberior team is happy to provide advice on labelling strategies and sample preparation for every approach – please don’t hesitate to get in touch!
For further details or to express your interest in learning how our systems can benefit your research, please send your information to Volodymyr (v.nechyporuk-zloy@imperial.ac.uk) or Andy (an-hill@photonlines.com).
We look forward to welcoming you to this exciting demonstration.