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@article{Li:2017:10.1074/mcp.RA117.000285,
author = {Li, Z and Gao, C and Zhang, Y and Palma, A and Childs, R and Silva, L and Liu, Y and Jiang, X and Liu, Y and Chai, W and Feizi, T},
doi = {10.1074/mcp.RA117.000285},
journal = {Molecular and Cellular Proteomics},
pages = {121--133},
title = {O-Glycome beam search arrays for carbohydrate ligand discovery},
url = {http://dx.doi.org/10.1074/mcp.RA117.000285},
volume = {17},
year = {2017}
}

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TY  - JOUR
AB  - O-glycosylation is a post-translational modification of proteins crucial to molecular mechanisms in health and disease. O-glycans are typically highly heterogeneous. The involvement of specific O-glycan sequences in many bio-recognition systems is yet to be determined due to a lack of efficient methodologies. We describe here a targeted microarray approach: O-glycome beam search that is both robust and efficient for O-glycan ligand-discovery. Substantial simplification of the complex O-glycome profile and facile chromatographic resolution is achieved by arraying O-glycans as branches, monitoring by mass spectrometry, focusing on promising fractions, and on-array immuno-sequencing. This is orders of magnitude more sensitive than traditional methods. We have applied beam search approach to porcine stomach mucin and identified extremely minor components previously undetected within the O-glycome of this mucin that are ligands for the adhesive proteins of two rotaviruses. The approach is applicable to O-glycome recognition studies in a wide range of biological settings to give insights into glycan recognition structures in natural microenvironments.
AU  - Li,Z
AU  - Gao,C
AU  - Zhang,Y
AU  - Palma,A
AU  - Childs,R
AU  - Silva,L
AU  - Liu,Y
AU  - Jiang,X
AU  - Liu,Y
AU  - Chai,W
AU  - Feizi,T
DO  - 10.1074/mcp.RA117.000285
EP  - 133
PY  - 2017///
SN  - 1535-9476
SP  - 121
TI  - O-Glycome beam search arrays for carbohydrate ligand discovery
T2  - Molecular and Cellular Proteomics
UR  - http://dx.doi.org/10.1074/mcp.RA117.000285
UR  - http://hdl.handle.net/10044/1/55198
VL  - 17
ER  -