In this section
@unpublished{Giotis:2019:10.1101/507467,
author = {Giotis, E and Carnell, G and Young, E and Ghanny, S and Soteropoulos, P and Barclay, W and Skinner, M and Temperton, N},
doi = {10.1101/507467},
title = {The MHC class-II HLA-DR receptor mediates bat influenza A-like H17N10 virus entry into mammalian cells},
url = {http://dx.doi.org/10.1101/507467},
year = {2019}
}
TY - UNPB
AB - Bats are notorious reservoirs of diverse, potentially zoonotic viruses, exemplified by the evolutionarily distinct, influenza A-like viruses H17N10 and H18N11 (BatIVs). The surface glycoproteins [haemagglutinin (H) and neuraminidase (N)] of BatIVs neither bind nor cleave sialic acid receptors, which suggests that these viruses employ cell attachment and entry mechanisms that differ from those of classical influenza A viruses (IAVs). Identifying the cellular factors that mediate entry and determine susceptibility to infection will help assess the host range of BatIVs. Here, we investigated a range of cell lines from different species for their susceptibility to infection by pseudotyped viruses bearing bat H17 and/or N10 envelope proteins. We show that a number of human haematopoietic cancer cell lines and the canine kidney MDCK II (but not MDCK I) cells are susceptible to H17-pseudotypes (H17-PV). We observed with microarrays and qRT-PCR that the dog leukocyte antigen DLA-DRA mRNA is overexpressed in late passaged parental MDCK and commercial MDCK II cells, compared to early passaged parental MDCK and MDCK I cells, respectively. The human orthologue HLA-DRA encodes the alpha subunit of the MHC class II HLA-DR antigen-binding heterodimer. Small interfering RNA- or neutralizing antibody-targeting HLA-DRA, drastically reduced the susceptibility of Raji B cells to H17-PV. Conversely, overexpression of HLA-DRA and its paralogue HLA-DRB1 on the surface of the unsusceptible HEK293T/17 cells conferred susceptibility to H17-PV. The identification of HLA-DR as an H17N10 entry mediator will contribute to a better understanding of the tropism of the virus and will elucidate its zoonotic transmission.
AU - Giotis,E
AU - Carnell,G
AU - Young,E
AU - Ghanny,S
AU - Soteropoulos,P
AU - Barclay,W
AU - Skinner,M
AU - Temperton,N
DO - 10.1101/507467
PY - 2019///
TI - The MHC class-II HLA-DR receptor mediates bat influenza A-like H17N10 virus entry into mammalian cells
UR - http://dx.doi.org/10.1101/507467
ER -