In this section
@article{Davies:2018:10.1101/395608,
author = {Davies, FJ and Olme, C and Lynskey, NN and Turner, CE and Sriskandan, S},
doi = {10.1101/395608},
title = {Streptococcal superantigen-induced expansion of human tonsil T cells leads to altered T follicular helper cell phenotype, B cell death, and reduced immunoglobulin release},
url = {http://dx.doi.org/10.1101/395608},
year = {2018}
}
TY - JOUR
AB - <jats:title>Abstract</jats:title><jats:sec><jats:title>Background</jats:title><jats:p>Streptococcal pyrogenic exotoxin (SPE)A expression is epidemiologically linked to streptococcal tonsillo-pharyngitis and outbreaks of scarlet fever, although the mechanisms by which superantigens confer advantage<jats:italic>Streptococcus pyogenes</jats:italic>are unclear.<jats:italic>S. pyogenes</jats:italic>is an exclusively human pathogen. As the leukocyte profile of tonsil differs from peripheral blood, the impact of SPEA production on human tonsil cell function was investigated.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>Human tonsil cells from routine tonsillectomy, were co-incubated with purified streptococcal superantigens or isogenic streptococcal culture supernatants, differing only in superantigen content. Tonsil cell proliferation was quantified by tritium-incorporation, and cell surface characteristics assessed by flow-cytometry. Soluble mediators were measured using ELISA and quantitative (q)RT-PCR was performed for immunoglobulin gene expression.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Tonsil T cells proliferated in response to SPEA and demonstrated typical release of pro-inflammatory cytokines. When cultured in the absence of superantigen, tonsil preparations released large quantities of immunoglobulin over 7d. In contrast, marked B cell apoptosis and abrogation of total IgA, IgM, and IgG production occurred in the presence of SPEA and other superantigens. In SPEA-stimulated cultures, T follicular helper (TfH) cells showed a reduction in CXCR5 expression, but up-regulation of CD134 (OX40), CD278 (ICOS) and CD150 (SLAM) expression, indicative of a phenotypic change in the TfH population and associated with impaired chemotactic response to CXCL13.</jats:p></jats:sec><ja
AU - Davies,FJ
AU - Olme,C
AU - Lynskey,NN
AU - Turner,CE
AU - Sriskandan,S
DO - 10.1101/395608
PY - 2018///
TI - Streptococcal superantigen-induced expansion of human tonsil T cells leads to altered T follicular helper cell phenotype, B cell death, and reduced immunoglobulin release
UR - http://dx.doi.org/10.1101/395608
UR - https://doi.org/10.1101/395608
ER -