In this section
@article{Thrupp:2020:10.1101/2020.04.13.035386,
author = {Thrupp, N and Frigerio, CS and Wolfs, L and Skene, NG and Poovathingal, S and Fourne, Y and Matthews, PM and Theys, T and Mancuso, R and de, Strooper B and Fiers, M},
doi = {10.1101/2020.04.13.035386},
title = {Single nucleus sequencing fails to detect microglial activation in human tissue},
url = {http://dx.doi.org/10.1101/2020.04.13.035386},
year = {2020}
}
TY - JOUR
AB - <jats:title>Abstract</jats:title><jats:p>Single nucleus RNA-Seq (snRNA-Seq) methods are used as an alternative to single cell RNA-Seq methods, as they allow transcriptomic profiling of frozen tissue. However, it is unclear whether snRNA-Seq is able to detect cellular state in human tissue. Indeed, snRNA-Seq analyses of human brain samples have failed to detect a consistent microglial activation signature in Alzheimer’s Disease. A comparison of microglia from single cells and single nuclei of four human subjects reveals that ~1% of genes is depleted in nuclei compared to whole cells. This small population contains 18% of genes previously implicated in microglial activation, including<jats:italic>APOE, CST3, FTL, SPP1</jats:italic>, and<jats:italic>CD74</jats:italic>. We confirm our findings across multiple previous single nucleus and single cell studies. Given the low sensitivity of snRNA-Seq to this population of activation genes, we conclude that snRNA-Seq is not suited to detecting cellular activation in microglia in human disease.</jats:p>
AU - Thrupp,N
AU - Frigerio,CS
AU - Wolfs,L
AU - Skene,NG
AU - Poovathingal,S
AU - Fourne,Y
AU - Matthews,PM
AU - Theys,T
AU - Mancuso,R
AU - de,Strooper B
AU - Fiers,M
DO - 10.1101/2020.04.13.035386
PY - 2020///
TI - Single nucleus sequencing fails to detect microglial activation in human tissue
UR - http://dx.doi.org/10.1101/2020.04.13.035386
UR - https://doi.org/10.1101/2020.04.13.035386
ER -