Citation

BibTex format

@article{Campbell:2020:10.4049/immunohorizons.2000068,
author = {Campbell, VL and Nguyen, L and Snoey, E and McClurkan, CL and Laing, KJ and Dong, L and Sette, A and Lindestam, Arlehamn CS and Altmann, DM and Boyton, RJ and Roby, JA and Gale, M and Stone, M and Busch, MP and Norris, PJ and Koelle, DM},
doi = {10.4049/immunohorizons.2000068},
journal = {ImmunoHorizons},
pages = {444--453},
title = {Proteome-wide Zika virus CD4 T cell epitope and HLA restriction determination},
url = {http://dx.doi.org/10.4049/immunohorizons.2000068},
volume = {4},
year = {2020}
}
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TY  - JOUR
AB  - Zika virus (ZIKV) is a mosquito-borne pathogen that caused an epidemic in 2015-2016. ZIKV-specific T cell responses are functional in animal infection models, and helper CD4 T cells promote avid Abs in the vaccine context. The small volumes of blood available from field research limit the determination of T cell epitopes for complex microbes such as ZIKV. The goal of this project was efficient determination of human ZIKV CD4 T cell epitopes at the whole proteome scale, including validation of reactivity to whole pathogen, using small blood samples from convalescent time points when T cell response magnitude may have waned. Polyclonal enrichment of candidate ZIKV-specific CD4 T cells used cell-associated virus, documenting that T cells in downstream peptide analyses also recognize whole virus after Ag processing. Sequential query of bulk ZIKV-reactive CD4 T cells with pooled/single ZIKV peptides and molecularly defined APC allowed precision epitope and HLA restriction assignments across the ZIKV proteome and enabled discovery of numerous novel ZIKV CD4 T cell epitopes. The research workflow is useful for the study of emerging infectious diseases with a very limited human blood sample availability.
AU  - Campbell,VL
AU  - Nguyen,L
AU  - Snoey,E
AU  - McClurkan,CL
AU  - Laing,KJ
AU  - Dong,L
AU  - Sette,A
AU  - Lindestam,Arlehamn CS
AU  - Altmann,DM
AU  - Boyton,RJ
AU  - Roby,JA
AU  - Gale,M
AU  - Stone,M
AU  - Busch,MP
AU  - Norris,PJ
AU  - Koelle,DM
DO  - 10.4049/immunohorizons.2000068
EP  - 453
PY  - 2020///
SN  - 2573-7732
SP  - 444
TI  - Proteome-wide Zika virus CD4 T cell epitope and HLA restriction determination
T2  - ImmunoHorizons
UR  - http://dx.doi.org/10.4049/immunohorizons.2000068
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32753403
VL  - 4
ER  -
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