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Journal articleChenine A-L, Merbah M, Wieczorek L, et al., 2018,
Neutralization sensitivity of a novel HIV-1 CRF01_AE panel of infectious molecular clones
, Journal of Acquired Immune Deficiency Syndromes, Vol: 78, Pages: 348-355, ISSN: 1525-4135BACKGROUND: HIV-1 CRF01_AE is dominant in Thailand where RV144 vaccine trial was conducted. To study immune correlates of protection in ongoing trials, CRF01_AE derived reagents are essential. Here we present a panel of 14 HIV-1 infectious molecular clones (IMC) identified from different stages of infection, and characterization of their neutralization sensitivity using two standard assays. METHODS: One full-length IMC was constructed using a transmitted-founder virus to express Renilla luciferase (LucR) reporter gene and full-length envelopes (envs) of exogenous HIV-1. A panel of IMCs was generated, expressing envs of viruses from acute (Fiebig stages I/II and I-IV) and chronic (>Febig VI) infection. Neutralization assays were performed using TZM-bl or A3R5 cell lines, and sera or monoclonal antibodies (mAbs). Wilcoxon matched-paired test was used to assess neutralization differences between assays and reagents; correlation coefficients were evaluated by linear regression. RESULTS: Neutralization potency observed was significantly higher in the A3R5 assay when testing mAbs and serum pools (p<0.0001); the stage of infection from which env was derived did not associate with IMC neutralization sensitivity. Neutralization values from A3R5 and TZM-bl assays were strongly correlated when mAbs were tested (R=0.7, p<0.0001), but a weaker association was seen with serum pools (R=0.17, p=0.03). CONCLUSIONS: This novel panel of CRF01_AE reporter-IMC is useful for assessing vaccine-induced neutralizing antibodies in multiple assays, including those utilizing primary cell targets. The significant differences in TZM-bl and A3R5 neutralization sensitivity, as well as the poor association when using polyclonal sera indicates the need for caution in choosing one specific platform.
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Journal articleShort CS, Quinlan R, Bennett P, et al., 2018,
Optimising the collection of female genital tract fluid for cytokine analysis in pregnant women
, Journal of Immunological Methods, Vol: 458, Pages: 15-20, ISSN: 0022-1759Introduction: To better understand the immunology of pregnancy, study of female genital tract fluid (FGF) is desirable. However the optimum method of collection of FGF in pregnant women for immunological methods, specifically cytokine measurement, is unknown.Methods:A prospective study of HIV-uninfected pregnant women comparing two methods of FGF collection: polyvinyl acetal sponge collection of cervical fluid (CF) and menstrual cup collection of cervicovaginal fluid (CVF). Samples were collected at 3 time points across the second and third trimesters: 14-21, 22-25 and 26-31 weeks. Multiplex chemi-luminescent assays were used to measure: IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13 and TNF-α. Optimal methodology for cytokine normalisation (sample weight, volume and total protein) was explored. ResultsAll cytokines were measurable in both fluid types. IL-1β, IL-8 and IL-6 were detected at the highest concentrations (ranking order CF > CVF > plasma). CVF collection was simpler, provided the largest volume of sample (median 0.5g) with the potential for undiluted usage, and allowed for self-insertion. CF cytokine concentrations were intrinsically associated with sample weight and protein concentration however CVF cytokines were independent of these. Conclusion:Both methods of collection are robust for measurement of FGF cytokines during pregnancy. We recommend CVF collection using a menstrual cup as a viable option in pregnant women for high dimensional biological techniques.
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Conference paperRiviere F, Dian C, Perez-Dorado I, et al., 2018,
Mechanistic insight into HsNMT1-mediated acylation
, Publisher: WILEY, Pages: 421-422, ISSN: 2211-5463 -
Conference paperChhabra S, Fidler S, Bower M, et al., 2018,
Malignancy and all-cause mortality: incidence in teenage young adults living with perinatally acquired HIV
, Publisher: JOHN WILEY & SONS LTD, Pages: 62-63 -
Journal article, 2018,
EASL Clinical Practice Guidelines: Management of alcohol-related liver disease
, JOURNAL OF HEPATOLOGY, Vol: 69, Pages: 154-181, ISSN: 0168-8278- Author Web Link
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- Citations: 535
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Journal articleBussel J, Arnold DM, Grossbard E, et al., 2018,
Fostamatinib for the treatment of adult persistent and chronic immune thrombocytopenia: Results of two phase 3, randomized, placebo-controlled trials
, American Journal of Hematology, Vol: 93, Pages: 921-930, ISSN: 0361-8609 -
Journal articleNagel M, Jansen PR, Stringer S, et al., 2018,
Meta-analysis of genome-wide association studies for neuroticism in 449,484 individuals identifies novel genetic loci and pathways
, NATURE GENETICS, Vol: 50, Pages: 920-+, ISSN: 1061-4036- Cite
- Citations: 467
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Journal articleZeis T, Howell OW, Reynolds R, et al., 2018,
Molecular pathology of Multiple Sclerosis lesions reveals a heterogeneous expression pattern of genes involved in oligodendrogliogenesis
, EXPERIMENTAL NEUROLOGY, Vol: 305, Pages: 76-88, ISSN: 0014-4886- Cite
- Citations: 24
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Conference paperTate EW, 2018,
Protein N terminal modifications: from chemical biology to drug discovery
, Publisher: WILEY, Pages: 72-73, ISSN: 2211-5463 -
Journal articleElson JL, Kochaj R, Reynolds R, et al., 2018,
Temporal-Spatial Profiling of Pedunculopontine Galanin-Cholinergic Neurons in the Lactacystin Rat Model of Parkinson's Disease
, NEUROTOXICITY RESEARCH, Vol: 34, Pages: 16-31, ISSN: 1029-8428- Author Web Link
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- Citations: 7
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