BibTex format
@article{Fowler:2022:10.7554/eLife.75046,
author = {Fowler, PW and Wright, C and Spiers, H and Zhu, T and Baeten, EML and Hoosdally, SW and Gibertoni, Cruz AL and Roohi, A and Kouchaki, S and Walker, TM and Peto, TEA and Miller, G and Lintott, C and Clifton, D and Crook, DW and Walker, AS and Zooniverse, Volunteer Community and CRyPTIC, Consortium},
doi = {10.7554/eLife.75046},
journal = {eLife},
title = {A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates},
url = {http://dx.doi.org/10.7554/eLife.75046},
volume = {11},
year = {2022}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - Tuberculosis is a respiratory disease that is treatable with antibiotics. An increasing prevalence of resistance means that to ensure a good treatment outcome it is desirable to test the susceptibility of each infection to different antibiotics. Conventionally, this is done by culturing a clinical sample and then exposing aliquots to a panel of antibiotics, each being present at a pre-determined concentration, thereby determining if the sample isresistant or susceptible to each sample. The minimum inhibitory concentration (MIC) of a drug is the lowestconcentration that inhibits growth and is a more useful quantity but requires each sample to be tested at a range ofconcentrations for each drug. Using 96-well broth micro dilution plates with each well containing a lyophilised pre-determined amount of an antibiotic is a convenient and cost-effective way to measure the MICs of several drugs at once for a clinical sample. Although accurate, this is still an expensive and slow process that requires highly-skilled and experienced laboratory scientists. Here we show that, through the BashTheBug project hosted on the Zooniverse citizen science platform, a crowd of volunteers can reproducibly and accurately determine the MICs for 13 drugs and that simply taking the median or mode of 11-17 independent classifications is sufficient. There is therefore a potential role for crowds to support (but not supplant) the role of experts in antibiotic susceptibility testing.
AU - Fowler,PW
AU - Wright,C
AU - Spiers,H
AU - Zhu,T
AU - Baeten,EML
AU - Hoosdally,SW
AU - Gibertoni,Cruz AL
AU - Roohi,A
AU - Kouchaki,S
AU - Walker,TM
AU - Peto,TEA
AU - Miller,G
AU - Lintott,C
AU - Clifton,D
AU - Crook,DW
AU - Walker,AS
AU - Zooniverse,Volunteer Community
AU - CRyPTIC,Consortium
DO - 10.7554/eLife.75046
PY - 2022///
SN - 2050-084X
TI - A crowd of BashTheBug volunteers reproducibly and accurately measure the minimum inhibitory concentrations of 13 antitubercular drugs from photographs of 96-well broth microdilution plates
T2 - eLife
UR - http://dx.doi.org/10.7554/eLife.75046
UR - https://www.ncbi.nlm.nih.gov/pubmed/35588296
VL - 11
ER -