BibTex format
@article{Martinson:2023:10.3390/vaccines11020334,
author = {Martinson, N and Gordhan, B and Petkov, S and Pillay, A-D and Seiphetlo, T and Singh, N and Otwombe, K and Lebina, L and Fredolini, C and Chiodi, F and Fox, J and Kana, B and Herrera, C},
doi = {10.3390/vaccines11020334},
journal = {Vaccines},
pages = {1--15},
title = {Proteomic analysis of mucosal and systemic responses to SARS-CoV-2 antigen},
url = {http://dx.doi.org/10.3390/vaccines11020334},
volume = {11},
year = {2023}
}
RIS format (EndNote, RefMan)
TY - JOUR
AB - The mucosal environment of the upper respiratory tract is the first barrier of protection against SARS-CoV-2 transmission. However, the mucosal factors involved in viral transmission and po-tentially modulating the capacity to prevent such transmission, have not fully been identified. In this pilot proteomics study, we compared mucosal and systemic compartments in a South African co-hort of vaccinated and unvaccinated individuals undergoing maxillofacial surgery with previous history of COVID-19 or not. Inflammatory profiles were analyzed in plasma, nasopharyngeal swabs, and nasal and oral tissue explant cultures, using Olink and Luminex technologies. SARS-CoV-2-specific antibody levels were measured in serum and tissue explants. An increased pro-inflammatory proteomic profile was measured in the nasal compartment compared to plasma. However, IP-10 and MIG levels were higher in secretions than in nasal tissue, and the opposite was observed for TGF-. Nasal anti-SARS-CoV-2 spike IgG correlated with mucosal MIG expression for all participants. A further positive correlation was found with IP-10 in BioNTech/Pfizer-vaccinated individuals. Systemic levels of anti-SARS-CoV-2 spike IgG elicited by this vaccine, correlated with plasma IL-10, IL-6 and HBD4. Proteomic profiles measured in mucosal tissues and secretions using combined technologies could reveal correlates of protection at the mucosal portals of viral entry.
AU - Martinson,N
AU - Gordhan,B
AU - Petkov,S
AU - Pillay,A-D
AU - Seiphetlo,T
AU - Singh,N
AU - Otwombe,K
AU - Lebina,L
AU - Fredolini,C
AU - Chiodi,F
AU - Fox,J
AU - Kana,B
AU - Herrera,C
DO - 10.3390/vaccines11020334
EP - 15
PY - 2023///
SN - 2076-393X
SP - 1
TI - Proteomic analysis of mucosal and systemic responses to SARS-CoV-2 antigen
T2 - Vaccines
UR - http://dx.doi.org/10.3390/vaccines11020334
UR - https://www.mdpi.com/2076-393X/11/2/334
VL - 11
ER -